Detailed information of TA system TA105880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1734782..1735269
• Replicon: chromosome
• Accession: NZ_CP029579
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: DLJ64_RS08955
• Protein ID: WP_001520461.1
• Coordinates: 1734782..1735033 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: DLJ64_RS08960
• Protein ID: WP_001520458.1
• Coordinates: 1735036..1735269 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS08930	1730394..1731680	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
DLJ64_RS08935	1731680..1732669	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
DLJ64_RS08940	1732666..1733487	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
DLJ64_RS08945	1733490..1733867	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
DLJ64_RS08950	1733874..1734722	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
DLJ64_RS08955	1734782..1735033	-	252	WP_001520461.1	CcdB family protein	Toxin
DLJ64_RS08960	1735036..1735269	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DLJ64_RS08965	1735355..1735834	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
DLJ64_RS08970	1736026..1737888	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
DLJ64_RS08975	1737881..1738411	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
DLJ64_RS08980	1738519..1739850	-	1332	WP_001520455.1	MFS transporter	-
DLJ64_RS08985	1739909..1740196	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T105880 WP_001520461.1 NZ_CP029579:c1735033-1734782 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T105880 NZ_CP029579:c1735033-1734782 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT105880 WP_001520458.1 NZ_CP029579:c1735269-1735036 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT105880 NZ_CP029579:c1735269-1735036 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4