Detailed information of TA system TA105864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 84420..84946
• Replicon: chromosome
• Accession: NZ_CP029579
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DLJ64_RS00480
• Protein ID: WP_000323025.1
• Coordinates: 84420..84707 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DLJ64_RS00485
• Protein ID: WP_000534858.1
• Coordinates: 84707..84946 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS00440	79444..79659	-	216	WP_000839590.1	phage lysis protein EssD	-
DLJ64_RS28995	79879..80049	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
DLJ64_RS00445	80413..80628	-	216	WP_000066484.1	cold shock-like protein CspB	-
DLJ64_RS00450	80929..81141	+	213	WP_000087756.1	cold shock-like protein CspF	-
DLJ64_RS28495	81196..81285	+	90	WP_120795389.1	hypothetical protein	-
DLJ64_RS00455	81563..82315	-	753	WP_001047135.1	antitermination protein	-
DLJ64_RS00460	82329..83378	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
DLJ64_RS00465	83380..83658	-	279	WP_012304870.1	hypothetical protein	-
DLJ64_RS00470	83725..83976	-	252	WP_000980994.1	hypothetical protein	-
DLJ64_RS00475	84193..84348	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
DLJ64_RS00480	84420..84707	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DLJ64_RS00485	84707..84946	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DLJ64_RS00490	84971..85276	+	306	WP_001326990.1	hypothetical protein	-
DLJ64_RS00495	85479..85811	+	333	WP_001301033.1	protein FlxA	-
DLJ64_RS00505	86248..87561	-	1314	WP_000589005.1	ISNCY family transposase	-
DLJ64_RS00510	88045..89073	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
DLJ64_RS00515	89070..89684	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	42388..102299	59911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T105864 WP_000323025.1 NZ_CP029579:c84707-84420 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T105864 NZ_CP029579:c84707-84420 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT105864 WP_000534858.1 NZ_CP029579:c84946-84707 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT105864 NZ_CP029579:c84946-84707 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8