Detailed information of TA system TA105858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 29748..30295
• Replicon: plasmid pDA33135-70
• Accession: NZ_CP029578
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A4Y1YQH8
• Locus tag: DLJ63_RS27280
• Protein ID: WP_012881118.1
• Coordinates: 30014..30295 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E2GHF7
• Locus tag: DLJ63_RS27275
• Protein ID: WP_012881119.1
• Coordinates: 29748..30017 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS27225	24964..25668	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DLJ63_RS27230	25723..26109	+	387	Protein_28	DUF2726 domain-containing protein	-
DLJ63_RS27235	26264..26737	+	474	WP_016240489.1	hypothetical protein	-
-	26880..26939	-	60	NuclAT_1	-	-
-	26880..26939	-	60	NuclAT_1	-	-
-	26880..26939	-	60	NuclAT_1	-	-
-	26880..26939	-	60	NuclAT_1	-	-
DLJ63_RS27245	26984..27133	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
DLJ63_RS27250	27418..27666	+	249	WP_000083839.1	replication regulatory protein RepA	-
DLJ63_RS27260	27911..27985	+	75	WP_001365705.1	RepA leader peptide Tap	-
DLJ63_RS27265	27978..28835	+	858	WP_162543890.1	incFII family plasmid replication initiator RepA	-
DLJ63_RS27275	29748..30017	+	270	WP_012881119.1	ABC transporter ATPase	Antitoxin
DLJ63_RS27280	30014..30295	+	282	WP_012881118.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DLJ63_RS27285	30341..31189	+	849	WP_012881117.1	3'-5' exonuclease	-
DLJ63_RS27290	31306..31788	+	483	WP_001311056.1	hypothetical protein	-
DLJ63_RS27295	31823..32929	-	1107	Protein_38	maltoporin LamB	-
DLJ63_RS27300	33381..34256	-	876	WP_001617865.1	class A extended-spectrum beta-lactamase CTX-M-14	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..70197	70197
	-	flank	IS/Tn	-	-	24964..25668	704
	-	flank	IS/Tn	blaCTX-M-14	-	33381..35768	2387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10875.53 Da
• Isoelectric Point: 8.4615

>T105858 WP_012881118.1 NZ_CP029578:30014-30295 [Escherichia coli]
MMEIFWTILASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T105858 NZ_CP029578:30014-30295 [Escherichia coli]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10206.54 Da
• Isoelectric Point: 8.8735

>AT105858 WP_012881119.1 NZ_CP029578:29748-30017 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EISRKVAGK

Nucleotide

• Length: 270 bp

>AT105858 NZ_CP029578:29748-30017 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A4Y1YQH8

Antitoxin

• AlphaFold DB: A0A4Y1YSD8