Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 100169..100694 Replicon plasmid pDA33135-139
Accession NZ_CP029577
Organism Escherichia coli strain DA33135

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DLJ63_RS26770 Protein ID WP_001159871.1
Coordinates 100389..100694 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DLJ63_RS26765 Protein ID WP_000813634.1
Coordinates 100169..100387 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ63_RS26755 97206..97847 - 642 WP_000990667.1 hypothetical protein -
DLJ63_RS26765 100169..100387 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DLJ63_RS26770 100389..100694 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DLJ63_RS26775 100695..101504 + 810 WP_000016979.1 site-specific integrase -
DLJ63_RS26780 101642..101917 - 276 WP_000239529.1 hypothetical protein -
DLJ63_RS26785 101911..102555 - 645 WP_000633911.1 AAA family ATPase -
DLJ63_RS26790 102784..103755 + 972 WP_001103694.1 hypothetical protein -
DLJ63_RS26795 103760..104152 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
DLJ63_RS26800 104157..104408 - 252 Protein_120 DUF4113 domain-containing protein -
DLJ63_RS26805 104470..105267 - 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / aadA5 / dfrA17 senB 1..139191 139191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T105853 WP_001159871.1 NZ_CP029577:100389-100694 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T105853 NZ_CP029577:100389-100694 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT105853 WP_000813634.1 NZ_CP029577:100169-100387 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT105853 NZ_CP029577:100169-100387 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References