Detailed information of TA system TA105849

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3856068..3856902
• Replicon: chromosome
• Accession: NZ_CP029576
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: DLJ63_RS20150
• Protein ID: WP_000854770.1
• Coordinates: 3856068..3856445 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: DLJ63_RS20155
• Protein ID: WP_001280950.1
• Coordinates: 3856534..3856902 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS20125	3852179..3853819	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
DLJ63_RS20130	3854592..3854768	-	177	Protein_3695	helix-turn-helix domain-containing protein	-
DLJ63_RS27820	3855132..3855290	-	159	WP_001467148.1	hypothetical protein	-
DLJ63_RS20140	3855390..3855566	-	177	WP_000839286.1	DUF957 domain-containing protein	-
DLJ63_RS20145	3855583..3856071	-	489	WP_000761690.1	hypothetical protein	-
DLJ63_RS20150	3856068..3856445	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
DLJ63_RS20155	3856534..3856902	-	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DLJ63_RS20160	3857065..3857286	-	222	WP_001220314.1	DUF987 domain-containing protein	-
DLJ63_RS20165	3857349..3857825	-	477	WP_001186779.1	RadC family protein	-
DLJ63_RS20170	3857841..3858305	-	465	WP_000855061.1	antirestriction protein	-
DLJ63_RS20180	3858647..3859465	-	819	WP_001234712.1	DUF945 domain-containing protein	-
DLJ63_RS20190	3859620..3859778	-	159	WP_001323397.1	DUF905 family protein	-
DLJ63_RS20195	3859849..3861750	-	1902	Protein_3706	Ag43/Cah family autotransporter adhesin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3844422..3884631	40209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T105849 WP_000854770.1 NZ_CP029576:c3856445-3856068 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T105849 NZ_CP029576:c3856445-3856068 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT105849 WP_001280950.1 NZ_CP029576:c3856902-3856534 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT105849 NZ_CP029576:c3856902-3856534 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885