Detailed information of TA system TA105848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3824699..3825519
• Replicon: chromosome
• Accession: NZ_CP029576
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: DLJ63_RS19990
• Protein ID: WP_001054379.1
• Coordinates: 3824699..3824956 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: DLJ63_RS19995
• Protein ID: WP_000123957.1
• Coordinates: 3824968..3825519 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS19955	3820179..3820682	-	504	Protein_3662	DNA helicase	-
DLJ63_RS19960	3820768..3821193	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
DLJ63_RS19965	3821190..3821540	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
DLJ63_RS19970	3821571..3823184	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
DLJ63_RS19975	3823214..3823945	-	732	Protein_3666	DNA helicase	-
DLJ63_RS19980	3824023..3824322	+	300	WP_001332034.1	GNAT family N-acetyltransferase	-
DLJ63_RS19990	3824699..3824956	+	258	WP_001054379.1	YjhX family toxin	Toxin
DLJ63_RS19995	3824968..3825519	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
DLJ63_RS20000	3825571..3826317	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
DLJ63_RS20005	3826445..3826705	+	261	WP_000077645.1	hypothetical protein	-
DLJ63_RS28000	3826743..3826859	+	117	Protein_3672	VOC family protein	-
DLJ63_RS20010	3827104..3828225	+	1122	WP_000010833.1	M42 family metallopeptidase	-
DLJ63_RS20015	3828222..3828500	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
DLJ63_RS20020	3828512..3829825	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T105848 WP_001054379.1 NZ_CP029576:3824699-3824956 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T105848 NZ_CP029576:3824699-3824956 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT105848 WP_000123957.1 NZ_CP029576:3824968-3825519 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT105848 NZ_CP029576:3824968-3825519 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030