Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3675501..3676051 Replicon chromosome
Accession NZ_CP029576
Organism Escherichia coli strain DA33135

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag DLJ63_RS19180 Protein ID WP_001160966.1
Coordinates 3675501..3675815 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag DLJ63_RS19185 Protein ID WP_000125566.1
Coordinates 3675818..3676051 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ63_RS19155 3671176..3672462 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
DLJ63_RS19160 3672462..3673451 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
DLJ63_RS19165 3673448..3674269 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
DLJ63_RS19170 3674272..3674649 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
DLJ63_RS19175 3674656..3675504 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
DLJ63_RS19180 3675501..3675815 - 315 WP_001160966.1 CcdB family protein Toxin
DLJ63_RS19185 3675818..3676051 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
DLJ63_RS19190 3676137..3676616 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
DLJ63_RS19195 3676808..3678670 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
DLJ63_RS19200 3678663..3679193 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
DLJ63_RS19205 3679301..3680632 - 1332 WP_001183207.1 MFS transporter -
DLJ63_RS19210 3680691..3680978 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T105846 WP_001160966.1 NZ_CP029576:c3675815-3675501 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T105846 NZ_CP029576:c3675815-3675501 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT105846 WP_000125566.1 NZ_CP029576:c3676051-3675818 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT105846 NZ_CP029576:c3676051-3675818 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References