Detailed information of TA system TA105841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2058920..2059446
• Replicon: chromosome
• Accession: NZ_CP029576
• Organism: Escherichia coli strain DA33135

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DLJ63_RS10645
• Protein ID: WP_000323025.1
• Coordinates: 2059159..2059446 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DLJ63_RS10640
• Protein ID: WP_000534858.1
• Coordinates: 2058920..2059159 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ63_RS10610	2054182..2054796	+	615	WP_001265627.1	hypothetical protein	-
DLJ63_RS10615	2054793..2055821	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
DLJ63_RS10620	2056305..2057618	+	1314	WP_000589005.1	ISNCY family transposase	-
DLJ63_RS10630	2058055..2058387	-	333	WP_001301033.1	protein FlxA	-
DLJ63_RS10635	2058590..2058895	-	306	WP_001326990.1	hypothetical protein	-
DLJ63_RS10640	2058920..2059159	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DLJ63_RS10645	2059159..2059446	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DLJ63_RS10650	2059518..2059673	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
DLJ63_RS10655	2059890..2060141	+	252	WP_000980994.1	hypothetical protein	-
DLJ63_RS10660	2060208..2060486	+	279	WP_012304870.1	hypothetical protein	-
DLJ63_RS10665	2060488..2061537	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
DLJ63_RS10670	2061551..2062303	+	753	WP_001047135.1	antitermination protein	-
DLJ63_RS27655	2062581..2062670	-	90	WP_120795389.1	hypothetical protein	-
DLJ63_RS10675	2062725..2062937	-	213	WP_000087756.1	cold shock-like protein CspF	-
DLJ63_RS10680	2063238..2063453	+	216	WP_000066484.1	cold shock-like protein CspB	-
DLJ63_RS28085	2063817..2063987	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
DLJ63_RS10685	2064207..2064422	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2033631..2095053	61422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T105841 WP_000323025.1 NZ_CP029576:2059159-2059446 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T105841 NZ_CP029576:2059159-2059446 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT105841 WP_000534858.1 NZ_CP029576:2058920-2059159 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT105841 NZ_CP029576:2058920-2059159 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8