105818

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33945..34470 Replicon plasmid pDA33133-157
Accession NZ_CP029575
Organism Escherichia coli strain DA33133

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DLJ62_RS24730 Protein ID WP_001159871.1
Coordinates 34165..34470 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag DLJ62_RS24725 Protein ID WP_000813630.1
Coordinates 33945..34163 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ62_RS24680 29950..30273 + 324 Protein_35 helix-turn-helix domain-containing protein -
DLJ62_RS25975 30275..30379 + 105 Protein_36 AAA family ATPase -
DLJ62_RS24690 30440..30953 + 514 Protein_37 DUF4113 domain-containing protein -
DLJ62_RS24695 31134..31831 - 698 Protein_38 IS1 family transposase -
DLJ62_RS24705 31888..32667 - 780 Protein_39 AAA family ATPase -
DLJ62_RS24710 32742..33158 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
DLJ62_RS24715 33155..33385 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DLJ62_RS24725 33945..34163 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DLJ62_RS24730 34165..34470 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DLJ62_RS24735 34471..35277 + 807 WP_000016970.1 site-specific integrase -
DLJ62_RS24740 35999..36754 + 756 WP_000852146.1 replication initiation protein RepE -
DLJ62_RS24745 37342..38508 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / blaCTX-M-15 / aac(3)-IId / mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(6')-Ib-cr / blaOXA-1 - 1..156518 156518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T105818 WP_001159871.1 NZ_CP029575:34165-34470 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T105818 NZ_CP029575:34165-34470 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT105818 WP_000813630.1 NZ_CP029575:33945-34163 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT105818 NZ_CP029575:33945-34163 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References