Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32742..33385 Replicon plasmid pDA33133-157
Accession NZ_CP029575
Organism Escherichia coli strain DA33133

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag DLJ62_RS24710 Protein ID WP_001034044.1
Coordinates 32742..33158 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag DLJ62_RS24715 Protein ID WP_001261286.1
Coordinates 33155..33385 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ62_RS24670 28227..29204 - 978 WP_000361611.1 RepB family plasmid replication initiator protein -
DLJ62_RS24680 29950..30273 + 324 Protein_35 helix-turn-helix domain-containing protein -
DLJ62_RS25975 30275..30379 + 105 Protein_36 AAA family ATPase -
DLJ62_RS24690 30440..30953 + 514 Protein_37 DUF4113 domain-containing protein -
DLJ62_RS24695 31134..31831 - 698 Protein_38 IS1 family transposase -
DLJ62_RS24705 31888..32667 - 780 Protein_39 AAA family ATPase -
DLJ62_RS24710 32742..33158 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
DLJ62_RS24715 33155..33385 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DLJ62_RS24725 33945..34163 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
DLJ62_RS24730 34165..34470 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
DLJ62_RS24735 34471..35277 + 807 WP_000016970.1 site-specific integrase -
DLJ62_RS24740 35999..36754 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / blaCTX-M-15 / aac(3)-IId / mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(6')-Ib-cr / blaOXA-1 - 1..156518 156518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T105817 WP_001034044.1 NZ_CP029575:c33158-32742 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T105817 NZ_CP029575:c33158-32742 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT105817 WP_001261286.1 NZ_CP029575:c33385-33155 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT105817 NZ_CP029575:c33385-33155 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References