Detailed information of TA system TA105801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1643405..1643981
• Replicon: chromosome
• Accession: NZ_CP029574
• Organism: Escherichia coli strain DA33133

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: DLJ62_RS08500
• Protein ID: WP_001297643.1
• Coordinates: 1643405..1643692 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: DLJ62_RS08505
• Protein ID: WP_000063156.1
• Coordinates: 1643679..1643981 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ62_RS08480	1639658..1640938	-	1281	WP_078207786.1	DUF445 domain-containing protein	-
DLJ62_RS08485	1640979..1642211	-	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
DLJ62_RS08490	1642357..1642452	-	96	Protein_1521	dienelactone hydrolase family protein	-
DLJ62_RS08495	1642509..1643206	+	698	Protein_1522	IS1 family transposase	-
DLJ62_RS08500	1643405..1643692	+	288	WP_001297643.1	BrnT family toxin	Toxin
DLJ62_RS08505	1643679..1643981	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
DLJ62_RS08510	1644015..1644971	-	957	WP_001297640.1	GTPase	-
DLJ62_RS08515	1644982..1645185	-	204	WP_000467859.1	YbdD/YjiX family protein	-
DLJ62_RS08520	1645235..1647385	-	2151	WP_001736090.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T105801 WP_001297643.1 NZ_CP029574:1643405-1643692 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T105801 NZ_CP029574:1643405-1643692 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT105801 WP_000063156.1 NZ_CP029574:1643679-1643981 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT105801 NZ_CP029574:1643679-1643981 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0