Detailed information of TA system TA105774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 804491..805062
• Replicon: chromosome
• Accession: NZ_CP029559
• Organism: Streptococcus sobrinus strain NIDR 6715-15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U2J9M5
• Locus tag: DLJ52_RS03965
• Protein ID: WP_021673296.1
• Coordinates: 804491..804823 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U2KH30
• Locus tag: DLJ52_RS03970
• Protein ID: WP_002959726.1
• Coordinates: 804817..805062 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ52_RS03950	799966..801579	+	1614	WP_109982536.1	alpha-glucosidase	-
DLJ52_RS03955	802128..802637	+	510	WP_019779284.1	DinB family protein	-
DLJ52_RS03960	802658..804007	+	1350	WP_109982537.1	replication-associated recombination protein A	-
DLJ52_RS03965	804491..804823	-	333	WP_021673296.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DLJ52_RS03970	804817..805062	-	246	WP_002959726.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DLJ52_RS03975	805213..805977	-	765	WP_019769317.1	DeoR/GlpR family DNA-binding transcription regulator	-
DLJ52_RS03980	806237..806665	+	429	WP_002959730.1	galactose-6-phosphate isomerase subunit LacA	-
DLJ52_RS03985	806693..807208	+	516	WP_002959732.1	galactose-6-phosphate isomerase subunit LacB	-
DLJ52_RS03990	807348..808328	+	981	WP_019795477.1	tagatose-bisphosphate aldolase	-
DLJ52_RS03995	808606..809439	+	834	WP_109982538.1	PRD domain-containing protein	-
DLJ52_RS04000	809476..809790	+	315	WP_002959739.1	PTS lactose/cellobiose transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12602.63 Da
• Isoelectric Point: 9.3092

>T105774 WP_021673296.1 NZ_CP029559:c804823-804491 [Streptococcus sobrinus]
MVEIKQGTIIKINLDPKEGHEQRGYRPYVCLSHSVVQNYSNIAIFAPISNTKRDYPFYVPLEGTKSTGKVLLDQPVTIDF
RARKYKFVEDIADSLLIELLSRVKVIFEKG

Nucleotide

• Length: 333 bp

>T105774 NZ_CP029559:c804823-804491 [Streptococcus sobrinus]
ATGGTAGAAATCAAGCAAGGAACTATAATAAAAATTAATCTAGACCCCAAGGAGGGACATGAACAAAGGGGCTATCGTCC
TTATGTCTGTTTGAGTCATAGTGTGGTGCAAAATTATTCCAATATAGCAATCTTTGCACCAATCAGTAATACCAAGCGTG
ACTACCCTTTTTATGTCCCCCTCGAAGGTACCAAATCAACTGGCAAAGTTTTACTTGACCAGCCGGTAACAATTGATTTC
AGAGCGAGAAAGTATAAATTCGTCGAAGATATTGCAGATTCTTTGCTCATTGAACTCCTATCACGTGTTAAAGTCATTTT
TGAGAAAGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9462.91 Da
• Isoelectric Point: 4.8146

>AT105774 WP_002959726.1 NZ_CP029559:c805062-804817 [Streptococcus sobrinus]
MPLTVNKWGNSIAIRLPKQLVQELKLQKDDILDYKISNNKLILEKKSEIPELTVEDLFKDYKGEPVDTTPFIFESKGNEQ
W

Nucleotide

• Length: 246 bp

>AT105774 NZ_CP029559:c805062-804817 [Streptococcus sobrinus]
ATGCCATTAACTGTCAATAAGTGGGGAAATAGTATTGCTATTCGTCTTCCCAAGCAATTAGTCCAAGAACTCAAACTACA
AAAAGATGATATTTTAGATTATAAAATATCAAATAATAAATTAATTTTAGAAAAAAAATCCGAAATTCCCGAATTAACTG
TTGAAGACCTTTTTAAGGACTACAAGGGAGAACCCGTAGATACGACTCCCTTTATTTTTGAGAGTAAGGGAAATGAACAA
TGGTAG

Structures

Toxin

• AlphaFold DB: U2J9M5

Antitoxin

• AlphaFold DB: U2KH30