Detailed information of TA system TA105770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5454653..5455364
• Replicon: chromosome
• Accession: NZ_CP029553
• Organism: Methylobacterium terrae strain 17Sr1-28

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DK419_RS25190
• Protein ID: WP_109961507.1
• Coordinates: 5455089..5455364 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: DK419_RS25185
• Protein ID: WP_109961506.1
• Coordinates: 5454653..5454967 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK419_RS25160	5450092..5451120	+	1029	WP_109961501.1	alpha/beta hydrolase	-
DK419_RS25165	5451176..5451685	-	510	WP_109961502.1	GNAT family N-acetyltransferase	-
DK419_RS25170	5451698..5452330	+	633	WP_109961503.1	hypothetical protein	-
DK419_RS28860	5452339..5452509	-	171	WP_162561398.1	hypothetical protein	-
DK419_RS25175	5452788..5454224	-	1437	WP_109961504.1	pyruvate kinase	-
DK419_RS25180	5454391..5454675	+	285	WP_109961505.1	BrnT family toxin	-
DK419_RS25185	5454653..5454967	+	315	WP_109961506.1	BrnA antitoxin family protein	Antitoxin
DK419_RS25190	5455089..5455364	+	276	WP_109961507.1	BrnT family toxin	Toxin
DK419_RS25195	5455351..5455671	+	321	WP_109961508.1	BrnA antitoxin family protein	-
DK419_RS25200	5455720..5456124	-	405	WP_109962495.1	DUF1036 domain-containing protein	-
DK419_RS25205	5456153..5457463	-	1311	WP_109961509.1	glycerate kinase	-
DK419_RS25210	5457536..5458492	-	957	WP_109961510.1	DMT family transporter	-
DK419_RS25215	5458656..5459423	+	768	WP_109961511.1	N-formylglutamate amidohydrolase	-
DK419_RS25220	5459479..5459784	+	306	WP_109961512.1	DUF1244 domain-containing protein	-
DK419_RS25225	5460033..5460305	+	273	WP_048429438.1	DUF2312 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-98)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10362.78 Da
• Isoelectric Point: 7.3161

>T105770 WP_109961507.1 NZ_CP029553:5455089-5455364 [Methylobacterium terrae]
MLFSYDEPKRQANLAKHGFDFAEFEEAFDFDRYAVLPAKPSHTGRARHKLVGTWYGVTVVMVIVSPLGSEATDIVSVRRA
DRKERTIYDAL

Nucleotide

• Length: 276 bp

>T105770 NZ_CP029553:5455089-5455364 [Methylobacterium terrae]
ATGCTGTTCAGCTACGACGAGCCGAAGCGGCAAGCGAATCTGGCAAAGCACGGCTTCGACTTCGCGGAGTTCGAGGAGGC
GTTCGATTTCGATCGCTACGCCGTCCTGCCGGCCAAGCCCAGCCATACCGGCCGCGCTCGGCACAAGCTGGTCGGCACGT
GGTACGGCGTGACCGTCGTCATGGTGATCGTCTCTCCGCTCGGCTCGGAGGCGACCGACATCGTCAGTGTCCGTCGTGCC
GACCGCAAGGAAAGGACCATCTATGACGCGCTCTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11496.99 Da
• Isoelectric Point: 4.9273

>AT105770 WP_109961506.1 NZ_CP029553:5454653-5454967 [Methylobacterium terrae]
MTGTAHPERPSLLTSEEDWDLDGEIPPLTAEELAQLRPARESLPPEVYAALPSRGGRPRSERPKQLVSLRLDPDVVETFK
ATGPGWQTRMNEVLAEAARKLRAA

Nucleotide

• Length: 315 bp

>AT105770 NZ_CP029553:5454653-5454967 [Methylobacterium terrae]
ATGACCGGTACTGCGCACCCTGAGCGGCCCTCCCTCCTCACCTCCGAGGAGGATTGGGACCTCGACGGCGAGATCCCGCC
TCTGACCGCCGAGGAACTGGCCCAGCTGCGCCCCGCCCGCGAGTCCCTTCCCCCCGAGGTCTACGCCGCGCTCCCGAGTC
GCGGCGGCCGACCGCGCTCCGAGCGGCCCAAGCAGCTCGTGAGCCTGCGCCTGGACCCGGACGTGGTCGAGACCTTCAAG
GCCACGGGGCCGGGCTGGCAGACGCGGATGAACGAGGTGCTGGCGGAAGCGGCGCGGAAGTTGCGGGCGGCCTAG