Detailed information of TA system TA105757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1671248..1671825
• Replicon: chromosome
• Accession: NZ_CP029544
• Organism: Lactobacillus helsingborgensis strain ESL0183

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DLD54_RS07995
• Protein ID: WP_109918860.1
• Coordinates: 1671493..1671825 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DLD54_RS07990
• Protein ID: WP_109918859.1
• Coordinates: 1671248..1671499 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLD54_RS07980	1668774..1669703	-	930	WP_109918857.1	cytochrome C5	-
DLD54_RS07985	1669853..1671091	+	1239	WP_109918858.1	MFS transporter	-
DLD54_RS07990	1671248..1671499	+	252	WP_109918859.1	hypothetical protein	Antitoxin
DLD54_RS07995	1671493..1671825	+	333	WP_109918860.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DLD54_RS08000	1672109..1673011	-	903	WP_109918861.1	LysR family transcriptional regulator	-
DLD54_RS08005	1673117..1674358	+	1242	WP_109918862.1	MFS transporter	-
DLD54_RS08010	1674363..1675802	+	1440	WP_109918863.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12707.77 Da
• Isoelectric Point: 9.0990

>T105757 WP_109918860.1 NZ_CP029544:1671493-1671825 [Lactobacillus helsingborgensis]
MVIKQGSILLMDLSPKQGHEQSGKQPYLYLSYKGVEKYAHIAVFAPISTTKRRYPLYQELPAASQTKGVVMLDQLVTIDY
HNQSFQYLEQLPDDYLEKILKIVKVVFQKD

Nucleotide

• Length: 333 bp

>T105757 NZ_CP029544:1671493-1671825 [Lactobacillus helsingborgensis]
ATGGTAATTAAGCAAGGATCTATTCTATTAATGGATTTAAGTCCTAAACAGGGGCATGAACAATCTGGCAAACAACCTTA
TTTATATTTAAGCTATAAAGGGGTTGAAAAATACGCTCATATTGCTGTGTTTGCTCCTATATCAACTACTAAAAGACGCT
ACCCCTTATATCAAGAATTACCTGCTGCATCTCAAACAAAAGGCGTAGTCATGCTAGATCAGCTAGTTACAATCGATTAT
CATAATCAATCATTTCAATATTTAGAACAACTACCTGATGACTATTTGGAAAAGATACTCAAAATTGTAAAAGTAGTTTT
TCAGAAAGATTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9591.75 Da
• Isoelectric Point: 4.4992

>AT105757 WP_109918859.1 NZ_CP029544:1671248-1671499 [Lactobacillus helsingborgensis]
MIRATISKWGNSKGIRLNQAVLQALNATIGDQLNFKIEDNKVIMSKQDDSPDLTFEELFKDYSGEEFDTEIKLPDERKGN
ERW

Nucleotide

• Length: 252 bp

>AT105757 NZ_CP029544:1671248-1671499 [Lactobacillus helsingborgensis]
ATGATTCGTGCTACTATATCTAAATGGGGTAATTCTAAAGGCATCCGATTAAACCAAGCAGTCTTGCAAGCATTAAACGC
TACTATAGGAGATCAGTTAAACTTTAAAATTGAGGACAACAAAGTAATAATGTCAAAGCAAGATGATTCACCTGATTTAA
CTTTTGAAGAACTTTTTAAAGATTATTCAGGTGAAGAATTTGATACCGAAATTAAGTTACCAGATGAGAGAAAAGGAAAT
GAAAGATGGTAA