105753

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 637622..638273 Replicon chromosome
Accession NZ_CP029536
Organism Lacticaseibacillus paracasei strain LC355

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag DKM32_RS03240 Protein ID WP_003567661.1
Coordinates 637622..638005 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag DKM32_RS03245 Protein ID WP_003567665.1
Coordinates 638025..638273 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKM32_RS03235 636917..637438 - 522 WP_003567657.1 QueT transporter family protein -
DKM32_RS03240 637622..638005 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DKM32_RS03245 638025..638273 - 249 WP_003567665.1 antitoxin Antitoxin
DKM32_RS03250 638341..639477 - 1137 WP_112199542.1 alanine racemase -
DKM32_RS03255 639464..639838 - 375 WP_112199543.1 holo-ACP synthase -
DKM32_RS03260 640008..641516 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
DKM32_RS16325 641642..641785 - 144 WP_003588978.1 hypothetical protein -
DKM32_RS03265 641816..643204 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T105753 WP_003567661.1 NZ_CP029536:c638005-637622 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T105753 NZ_CP029536:c638005-637622 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAGGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT105753 WP_003567665.1 NZ_CP029536:c638273-638025 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT105753 NZ_CP029536:c638273-638025 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCCGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References