Detailed information of TA system TA105752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 144145..144943
• Replicon: plasmid pHS30-1
• Accession: NZ_CP029493
• Organism: Escherichia coli strain HS30-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: DKQ63_RS23815
• Protein ID: WP_072662620.1
• Coordinates: 144422..144943 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: DKQ63_RS23810
• Protein ID: WP_001711191.1
• Coordinates: 144145..144414 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKQ63_RS23775	139306..139500	+	195	Protein_152	iron-containing alcohol dehydrogenase	-
DKQ63_RS23780	139655..140389	-	735	WP_000578338.1	helix-turn-helix domain-containing protein	-
DKQ63_RS23785	140479..140574	+	96	Protein_154	PerC family transcriptional regulator	-
DKQ63_RS23795	141981..142748	+	768	WP_001711193.1	hypothetical protein	-
DKQ63_RS23800	142801..143154	-	354	WP_161953766.1	hypothetical protein	-
DKQ63_RS23805	143160..143825	-	666	WP_023135658.1	AAA family ATPase	-
DKQ63_RS23810	144145..144414	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
DKQ63_RS23815	144422..144943	+	522	WP_072662620.1	GNAT family N-acetyltransferase	Toxin
DKQ63_RS24235	145009..145152	+	144	WP_162618456.1	hypothetical protein	-
DKQ63_RS23825	145167..145475	-	309	Protein_162	DDE-type integrase/transposase/recombinase	-
DKQ63_RS23830	145492..146568	-	1077	WP_038989268.1	IS110-like element ISEc21 family transposase	-
DKQ63_RS23840	146850..147446	-	597	Protein_164	IS3 family transposase	-
DKQ63_RS23845	147511..148514	+	1004	Protein_165	IS30-like element IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aadA2 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / tet(A) / mcr-1.1 / floR / blaTEM-1B	-	1..179444	179444
	-	inside	IScluster/Tn	-	-	137472..150242	12770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19359.18 Da
• Isoelectric Point: 8.2462

>T105752 WP_072662620.1 NZ_CP029493:144422-144943 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T105752 NZ_CP029493:144422-144943 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT105752 WP_001711191.1 NZ_CP029493:144145-144414 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT105752 NZ_CP029493:144145-144414 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2