Detailed information of TA system TA105751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 130821..131422
• Replicon: plasmid pHS30-1
• Accession: NZ_CP029493
• Organism: Escherichia coli strain HS30-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DKQ63_RS23695
• Protein ID: WP_001216039.1
• Coordinates: 130821..131201 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: DKQ63_RS23700
• Protein ID: WP_001190711.1
• Coordinates: 131201..131422 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKQ63_RS23655	125940..126644	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DKQ63_RS23665	126699..127079	+	381	Protein_134	recombinase family protein	-
DKQ63_RS23670	127262..128122	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
DKQ63_RS23675	128343..129040	-	698	Protein_136	IS1 family transposase	-
DKQ63_RS23685	129119..130585	+	1467	WP_074139093.1	PAS domain-containing methyl-accepting chemotaxis protein	-
DKQ63_RS23695	130821..131201	-	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DKQ63_RS23700	131201..131422	-	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DKQ63_RS23710	131687..132384	+	698	Protein_140	IS1 family transposase	-
DKQ63_RS23715	132401..132727	-	327	Protein_141	hypothetical protein	-
DKQ63_RS23720	132800..134062	-	1263	WP_069067570.1	hypothetical protein	-
DKQ63_RS23730	134364..135065	-	702	WP_104730539.1	hypothetical protein	-
DKQ63_RS23735	135062..135739	-	678	Protein_144	metallophosphoesterase	-
DKQ63_RS23740	135736..136362	-	627	WP_000484116.1	norphogenetic protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aadA2 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / tet(A) / mcr-1.1 / floR / blaTEM-1B	-	1..179444	179444
	-	inside	IScluster/Tn	floR / blaTEM-1B	-	118712..132384	13672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T105751 WP_001216039.1 NZ_CP029493:c131201-130821 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T105751 NZ_CP029493:c131201-130821 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT105751 WP_001190711.1 NZ_CP029493:c131422-131201 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT105751 NZ_CP029493:c131422-131201 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA