Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71416..72059 Replicon plasmid pHS30-1
Accession NZ_CP029493
Organism Escherichia coli strain HS30-1

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag DKQ63_RS23310 Protein ID WP_000754566.1
Coordinates 71416..71832 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag DKQ63_RS23315 Protein ID WP_001261276.1
Coordinates 71829..72059 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKQ63_RS23285 66729..67136 - 408 WP_001214976.1 cysteine hydrolase -
DKQ63_RS23290 67125..68876 + 1752 Protein_74 Tn3-like element TnAs1 family transposase -
DKQ63_RS23295 68997..70073 - 1077 WP_000227969.1 IS110 family transposase -
DKQ63_RS23305 70355..71211 - 857 Protein_76 IS3 family transposase -
DKQ63_RS23310 71416..71832 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
DKQ63_RS23315 71829..72059 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DKQ63_RS23325 72258..72749 - 492 Protein_79 IS5-like element ISKpn26 family transposase -
DKQ63_RS23330 72855..73559 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
DKQ63_RS23340 73613..73822 + 210 Protein_81 IS110 family transposase -
DKQ63_RS23345 74099..74574 - 476 Protein_82 IS1 family transposase -
DKQ63_RS23350 74967..75995 + 1029 WP_054528934.1 tyrosine-type recombinase/integrase -
DKQ63_RS23355 76018..76908 - 891 Protein_84 IS30-like element IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aadA2 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / tet(A) / mcr-1.1 / floR / blaTEM-1B - 1..179444 179444
- inside IScluster/Tn aph(6)-Id / aph(3'')-Ib / sul2 / aadA2 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / tet(A) / mcr-1.1 - 31049..99275 68226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T105749 WP_000754566.1 NZ_CP029493:c71832-71416 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T105749 NZ_CP029493:c71832-71416 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT105749 WP_001261276.1 NZ_CP029493:c72059-71829 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT105749 NZ_CP029493:c72059-71829 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References