Detailed information of TA system TA105728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1344009..1344580
• Replicon: chromosome
• Accession: NZ_CP029491
• Organism: Streptococcus sobrinus strain 10919

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DK182_RS06615
• Protein ID: WP_019775278.1
• Coordinates: 1344248..1344580 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U2KH30
• Locus tag: DK182_RS06610
• Protein ID: WP_002959726.1
• Coordinates: 1344009..1344254 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK182_RS06580	1339282..1339596	-	315	WP_002959739.1	PTS lactose/cellobiose transporter subunit IIA	-
DK182_RS06585	1339633..1340466	-	834	WP_019770295.1	PRD domain-containing protein	-
DK182_RS06590	1340744..1341724	-	981	WP_002959735.1	tagatose-bisphosphate aldolase	-
DK182_RS06595	1341864..1342379	-	516	WP_002959732.1	galactose-6-phosphate isomerase subunit LacB	-
DK182_RS06600	1342407..1342835	-	429	WP_002959730.1	galactose-6-phosphate isomerase subunit LacA	-
DK182_RS06605	1343095..1343858	+	764	Protein_1229	DeoR/GlpR transcriptional regulator	-
DK182_RS06610	1344009..1344254	+	246	WP_002959726.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DK182_RS06615	1344248..1344580	+	333	WP_019775278.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DK182_RS06620	1345064..1346413	-	1350	WP_109833611.1	replication-associated recombination protein A	-
DK182_RS06625	1346434..1346943	-	510	WP_109833612.1	DinB family protein	-
DK182_RS06630	1347492..1349105	-	1614	WP_019777149.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12618.67 Da
• Isoelectric Point: 9.3092

>T105728 WP_019775278.1 NZ_CP029491:1344248-1344580 [Streptococcus sobrinus]
MVEIKQGTIIKINLDPKEGHEQRGYRPYVCLSHSVVQNYSNIAIFAPISNTKRDYPFYVPLEGTKSTGKVLLDQLVTIDF
RARKYKFVEDIADSLLIELLSRVKVIFEKG

Nucleotide

• Length: 333 bp

>T105728 NZ_CP029491:1344248-1344580 [Streptococcus sobrinus]
ATGGTAGAAATCAAGCAAGGAACTATAATAAAAATTAATCTAGACCCCAAGGAGGGACATGAACAAAGGGGCTATCGTCC
TTATGTCTGTTTGAGTCATAGTGTGGTGCAAAATTATTCCAATATAGCAATCTTTGCACCAATCAGTAATACCAAGCGTG
ACTACCCTTTTTATGTCCCCCTCGAAGGTACCAAATCAACTGGCAAAGTTTTACTTGACCAGCTGGTAACAATTGATTTC
AGAGCGAGAAAGTATAAATTCGTCGAAGATATTGCAGATTCTTTGCTCATTGAACTCCTATCACGTGTTAAAGTCATTTT
TGAGAAAGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9462.91 Da
• Isoelectric Point: 4.8146

>AT105728 WP_002959726.1 NZ_CP029491:1344009-1344254 [Streptococcus sobrinus]
MPLTVNKWGNSIAIRLPKQLVQELKLQKDDILDYKISNNKLILEKKSEIPELTVEDLFKDYKGEPVDTTPFIFESKGNEQ
W

Nucleotide

• Length: 246 bp

>AT105728 NZ_CP029491:1344009-1344254 [Streptococcus sobrinus]
ATGCCATTAACTGTCAATAAGTGGGGAAATAGTATTGCTATTCGTCTTCCCAAGCAATTAGTCCAAGAACTCAAACTACA
AAAAGATGATATTTTAGATTATAAAATATCAAATAATAAATTAATTTTAGAAAAAAAATCCGAAATTCCCGAATTAACTG
TTGAAGACCTTTTTAAGGACTACAAGGGAGAACCCGTAGATACGACTCCCTTTATTTTTGAGAGTAAGGGAAATGAACAA
TGGTAG

Structures

Antitoxin

• AlphaFold DB: U2KH30