Detailed information of TA system TA105694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1450306..1450943
• Replicon: chromosome
• Accession: NZ_CP029473
• Organism: Bacillus velezensis strain Hx05

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: B7L90_RS06960
• Protein ID: WP_003156187.1
• Coordinates: 1450306..1450656 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: B7L90_RS06965
• Protein ID: WP_003156188.1
• Coordinates: 1450662..1450943 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L90_RS06920	1445346..1445948	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
B7L90_RS06925	1445948..1446736	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
B7L90_RS06930	1446702..1447184	-	483	WP_119891451.1	anti-sigma B factor RsbW	-
B7L90_RS06935	1447181..1447510	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
B7L90_RS06940	1447574..1448581	-	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
B7L90_RS06945	1448593..1448994	-	402	WP_003156178.1	anti-sigma regulatory factor	-
B7L90_RS06950	1448997..1449362	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
B7L90_RS06955	1449367..1450188	-	822	WP_003156182.1	STAS domain-containing protein	-
B7L90_RS06960	1450306..1450656	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
B7L90_RS06965	1450662..1450943	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
B7L90_RS06970	1451063..1452232	-	1170	WP_025853726.1	alanine racemase	-
B7L90_RS06975	1452349..1453356	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
B7L90_RS06980	1453520..1453885	-	366	WP_014304402.1	holo-ACP synthase	-
B7L90_RS06985	1453978..1454577	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T105694 WP_003156187.1 NZ_CP029473:c1450656-1450306 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T105694 NZ_CP029473:c1450656-1450306 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT105694 WP_003156188.1 NZ_CP029473:c1450943-1450662 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT105694 NZ_CP029473:c1450943-1450662 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5