Detailed information of TA system TA105691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1357143..1358060
• Replicon: chromosome
• Accession: NZ_CP029466
• Organism: Bacillus amyloliquefaciens strain HM618

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DKG78_RS07130
• Protein ID: WP_052827326.1
• Coordinates: 1357314..1358060 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: DKG78_RS07125
• Protein ID: WP_003154807.1
• Coordinates: 1357143..1357313 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKG78_RS07090	1352356..1353945	+	1590	WP_132106180.1	hypothetical protein	-
DKG78_RS07095	1353958..1354383	+	426	WP_132106178.1	hypothetical protein	-
DKG78_RS07100	1354388..1354585	+	198	WP_007610833.1	XkdX family protein	-
DKG78_RS07105	1354642..1355403	+	762	WP_076423976.1	phage portal protein	-
DKG78_RS07110	1355455..1355718	+	264	WP_003154815.1	hemolysin XhlA family protein	-
DKG78_RS07115	1355732..1355995	+	264	WP_003154813.1	phage holin	-
DKG78_RS07120	1356009..1356887	+	879	WP_129004094.1	N-acetylmuramoyl-L-alanine amidase	-
DKG78_RS07125	1357143..1357313	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
DKG78_RS07130	1357314..1358060	-	747	WP_052827326.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DKG78_RS07135	1358165..1359163	-	999	WP_132106176.1	inorganic phosphate transporter	-
DKG78_RS07140	1359176..1359793	-	618	WP_003154804.1	DUF47 domain-containing protein	-
DKG78_RS07145	1360079..1361395	-	1317	WP_007610842.1	amino acid permease	-
DKG78_RS07150	1361718..1362668	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29067.53 Da
• Isoelectric Point: 4.7755

>T105691 WP_052827326.1 NZ_CP029466:c1358060-1357314 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDSESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T105691 NZ_CP029466:c1358060-1357314 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATTCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGATGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT105691 WP_003154807.1 NZ_CP029466:c1357313-1357143 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT105691 NZ_CP029466:c1357313-1357143 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14