Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 513867..514503 Replicon chromosome
Accession NZ_CP029465
Organism Bacillus inaquosorum strain KCTC 13429

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DKG76_RS02625 Protein ID WP_003156187.1
Coordinates 514153..514503 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag DKG76_RS02620 Protein ID WP_003225183.1
Coordinates 513867..514148 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKG76_RS02600 510228..510827 - 600 WP_003240338.1 rhomboid family intramembrane serine protease -
DKG76_RS02605 510922..511287 + 366 WP_003240339.1 holo-ACP synthase -
DKG76_RS02610 511452..512468 + 1017 WP_080030878.1 outer membrane lipoprotein carrier protein LolA -
DKG76_RS02615 512583..513752 + 1170 WP_003240348.1 alanine racemase -
DKG76_RS02620 513867..514148 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DKG76_RS02625 514153..514503 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DKG76_RS02630 514619..515443 + 825 WP_003240350.1 RsbT co-antagonist protein RsbRA -
DKG76_RS02635 515448..515813 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
DKG76_RS02640 515817..516218 + 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
DKG76_RS02645 516230..517237 + 1008 WP_003240354.1 phosphoserine phosphatase RsbU -
DKG76_RS02650 517299..517628 + 330 WP_003240357.1 anti-sigma factor antagonist RsbV -
DKG76_RS02655 517625..518107 + 483 WP_003240359.1 anti-sigma B factor RsbW -
DKG76_RS02660 518073..518861 + 789 WP_003240361.1 RNA polymerase sigma factor SigB -
DKG76_RS02665 518861..519460 + 600 WP_003240363.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T105688 WP_003156187.1 NZ_CP029465:514153-514503 [Bacillus inaquosorum]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T105688 NZ_CP029465:514153-514503 [Bacillus inaquosorum]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT105688 WP_003225183.1 NZ_CP029465:513867-514148 [Bacillus inaquosorum]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT105688 NZ_CP029465:513867-514148 [Bacillus inaquosorum]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References