Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3367781..3368417 Replicon chromosome
Accession NZ_CP029464
Organism Bacillus pumilus strain ZB201701

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag DKE43_RS17340 Protein ID WP_003214169.1
Coordinates 3368067..3368417 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag DKE43_RS17335 Protein ID WP_012008995.1
Coordinates 3367781..3368062 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKE43_RS17315 3363927..3364532 - 606 WP_060595589.1 rhomboid family intramembrane serine protease -
DKE43_RS17320 3364627..3364992 + 366 WP_034663960.1 holo-ACP synthase -
DKE43_RS17325 3365153..3366169 + 1017 WP_106037124.1 outer membrane lipoprotein carrier protein LolA -
DKE43_RS17330 3366306..3367487 + 1182 WP_066031291.1 alanine racemase -
DKE43_RS17335 3367781..3368062 + 282 WP_012008995.1 hypothetical protein Antitoxin
DKE43_RS17340 3368067..3368417 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DKE43_RS17345 3368535..3369410 + 876 WP_045210228.1 RsbT co-antagonist protein RsbRA -
DKE43_RS17350 3369370..3369738 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
DKE43_RS17355 3369741..3370142 + 402 WP_034663954.1 anti-sigma regulatory factor -
DKE43_RS17360 3370153..3371160 + 1008 WP_095286219.1 PP2C family protein-serine/threonine phosphatase -
DKE43_RS17365 3371220..3371549 + 330 WP_034663950.1 anti-sigma factor antagonist -
DKE43_RS17370 3371546..3372034 + 489 WP_034663948.1 anti-sigma B factor RsbW -
DKE43_RS17375 3372000..3372788 + 789 WP_034663946.1 RNA polymerase sigma factor SigB -
DKE43_RS17380 3372788..3373387 + 600 WP_034663944.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T105687 WP_003214169.1 NZ_CP029464:3368067-3368417 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T105687 NZ_CP029464:3368067-3368417 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATCAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT105687 WP_012008995.1 NZ_CP029464:3367781-3368062 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT105687 NZ_CP029464:3367781-3368062 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References