Detailed information of TA system TA105686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 220914..221386
• Replicon: chromosome
• Accession: NZ_CP029462
• Organism: Megasphaera stantonii strain AJH120

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DKB62_RS01000
• Protein ID: WP_107196109.1
• Coordinates: 220914..221111 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DKB62_RS01005
• Protein ID: WP_107196108.1
• Coordinates: 221108..221386 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKB62_RS00980	217413..218963	+	1551	WP_087477024.1	polysaccharide biosynthesis protein	-
DKB62_RS00990	219825..220220	-	396	WP_157949714.1	HEPN domain-containing protein	-
DKB62_RS00995	220213..220554	-	342	WP_107196110.1	nucleotidyltransferase domain-containing protein	-
DKB62_RS01000	220914..221111	-	198	WP_107196109.1	type II toxin-antitoxin system YafQ family toxin	Toxin
DKB62_RS01005	221108..221386	-	279	WP_107196108.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DKB62_RS01010	221678..222025	-	348	WP_107196107.1	type II secretion system GspH family protein	-
DKB62_RS01015	222143..222469	-	327	WP_107196106.1	hypothetical protein	-
DKB62_RS01020	222665..222886	+	222	WP_107196105.1	hypothetical protein	-
DKB62_RS01025	222943..223134	-	192	WP_107196131.1	transposon-encoded TnpW family protein	-
DKB62_RS01030	223178..223819	-	642	WP_107196104.1	hypothetical protein	-
DKB62_RS12455	224372..224719	-	348	WP_157949713.1	hypothetical protein	-
DKB62_RS01045	225259..225894	-	636	WP_107196101.1	flavodoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(W)	-	219825..255809	35984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-65)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-90)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7592.99 Da
• Isoelectric Point: 9.8374

>T105686 WP_107196109.1 NZ_CP029462:c221111-220914 [Megasphaera stantonii]
MTYELVTTAKFRKDIKLMKKRGKNLKLLQEVLDTLLEGKLLDAKYKDHALVGNYIGFRECHIQPD

Nucleotide

• Length: 198 bp

>T105686 NZ_CP029462:c221111-220914 [Megasphaera stantonii]
ATGACGTATGAATTAGTCACGACTGCAAAATTCCGCAAAGATATCAAACTCATGAAGAAGCGCGGCAAAAATTTGAAGCT
GCTGCAGGAGGTTCTCGATACATTATTGGAAGGGAAGCTGTTGGATGCAAAATATAAAGACCATGCACTGGTAGGAAATT
ATATTGGCTTTCGCGAATGTCATATACAGCCGGATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10148.70 Da
• Isoelectric Point: 5.1369

>AT105686 WP_107196108.1 NZ_CP029462:c221386-221108 [Megasphaera stantonii]
MAKTATINIRIEPEIKADVEKLFSSFGITVSDAVNIFLRKSLMEGGLPFDVKQPRYNAETEAAMQEARNIAAGMVPAKRY
ANAKELFEDLGV

Nucleotide

• Length: 279 bp

>AT105686 NZ_CP029462:c221386-221108 [Megasphaera stantonii]
ATGGCCAAAACAGCGACAATTAATATACGAATTGAGCCAGAAATTAAAGCCGATGTAGAAAAGCTATTTTCCAGCTTTGG
GATTACCGTCAGCGACGCCGTAAATATCTTTTTGCGCAAATCGTTGATGGAAGGCGGGCTGCCCTTTGACGTGAAACAGC
CGCGGTATAATGCGGAAACCGAAGCGGCTATGCAGGAAGCTAGGAACATTGCGGCAGGAATGGTTCCGGCAAAGCGGTAT
GCGAATGCGAAAGAATTATTTGAGGACTTGGGCGTATGA