Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 427378..428014 Replicon chromosome
Accession NZ_CP029461
Organism Bacillus subtilis strain QB61

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DJ572_RS02175 Protein ID WP_003156187.1
Coordinates 427664..428014 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag DJ572_RS02170 Protein ID WP_003225183.1
Coordinates 427378..427659 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DJ572_RS02150 423738..424337 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
DJ572_RS02155 424432..424797 + 366 WP_015252768.1 holo-ACP synthase -
DJ572_RS02160 424963..425979 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
DJ572_RS02165 426093..427262 + 1170 WP_119899042.1 alanine racemase -
DJ572_RS02170 427378..427659 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DJ572_RS02175 427664..428014 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DJ572_RS02180 428129..428953 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
DJ572_RS02185 428958..429323 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
DJ572_RS02190 429327..429728 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
DJ572_RS02195 429740..430747 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
DJ572_RS02200 430809..431138 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
DJ572_RS02205 431135..431617 + 483 WP_003234299.1 anti-sigma B factor RsbW -
DJ572_RS02210 431583..432371 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
DJ572_RS02215 432371..432970 + 600 WP_095252677.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T105684 WP_003156187.1 NZ_CP029461:427664-428014 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T105684 NZ_CP029461:427664-428014 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT105684 WP_003225183.1 NZ_CP029461:427378-427659 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT105684 NZ_CP029461:427378-427659 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References