Detailed information of TA system TA105680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 911819..912480
• Replicon: plasmid pSF25509b
• Accession: NZ_CP029452
• Organism: Sinorhizobium fredii CCBAU 25509

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2Z3GJ97
• Locus tag: AOX55_RS25145
• Protein ID: WP_037400257.1
• Coordinates: 911819..912256 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2Z3GA74
• Locus tag: AOX55_RS25150
• Protein ID: WP_037400254.1
• Coordinates: 912253..912480 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX55_RS25110	906838..907263	+	426	WP_037400278.1	VOC family protein	-
AOX55_RS25115	907431..908681	-	1251	WP_037400275.1	bifunctional alpha/beta hydrolase/OsmC family protein	-
AOX55_RS25120	908908..909414	-	507	WP_037400273.1	peptide-methionine (S)-S-oxide reductase MsrA	-
AOX55_RS25125	909415..909858	-	444	WP_037400270.1	peptide-methionine (R)-S-oxide reductase MsrB	-
AOX55_RS25130	910105..910560	-	456	WP_037400267.1	carboxymuconolactone decarboxylase family protein	-
AOX55_RS25135	910587..911006	-	420	WP_037400264.1	cupin domain-containing protein	-
AOX55_RS25140	911246..911779	+	534	WP_037400260.1	Rrf2 family transcriptional regulator	-
AOX55_RS25145	911819..912256	-	438	WP_037400257.1	PIN domain-containing protein	Toxin
AOX55_RS25150	912253..912480	-	228	WP_037400254.1	hypothetical protein	Antitoxin
AOX55_RS25155	912921..913877	+	957	WP_037400251.1	patatin-like phospholipase family protein	-
AOX55_RS25165	914314..915705	-	1392	WP_037400248.1	S8 family serine peptidase	-
AOX55_RS25170	915848..916390	+	543	WP_037400245.1	RNA polymerase sigma factor	-
AOX55_RS25175	916387..917169	+	783	WP_109982249.1	anti-sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / katA	1..2210745	2210745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15263.44 Da
• Isoelectric Point: 6.4801

>T105680 WP_037400257.1 NZ_CP029452:c912256-911819 [Sinorhizobium fredii CCBAU 25509]
VTFLLDVNVLIALIDPGHVAHDDAHDWFASTGQTAWATCPITENGVLRIVGNPKYPNSPGSPALVMEIVGKLRSLPGHCF
WPDDVSLVGSGDIAPAKILTSGQVTDTYLLALAKAHDGQLATFDRKLTAGAVTRGNSALHLITGK

Nucleotide

• Length: 438 bp

>T105680 NZ_CP029452:c912256-911819 [Sinorhizobium fredii CCBAU 25509]
GTGACCTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGGGCCATGTAGCCCATGATGATGCGCACGATTG
GTTCGCATCGACCGGCCAGACCGCGTGGGCGACCTGTCCCATTACCGAAAACGGTGTCCTCCGCATTGTCGGCAATCCCA
AATACCCCAATTCTCCCGGCTCACCGGCACTCGTGATGGAGATCGTTGGCAAGTTGCGGTCGCTCCCTGGTCATTGTTTT
TGGCCGGACGACGTGAGCCTCGTCGGGTCAGGTGATATCGCTCCCGCGAAAATCCTGACTTCGGGGCAGGTAACCGATAC
CTATCTGCTTGCGCTCGCGAAGGCGCATGACGGCCAGTTGGCGACATTCGATCGCAAGCTAACAGCAGGGGCGGTCACGA
GGGGCAACTCTGCCTTGCACCTGATCACCGGCAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8111.34 Da
• Isoelectric Point: 9.1347

>AT105680 WP_037400254.1 NZ_CP029452:c912480-912253 [Sinorhizobium fredii CCBAU 25509]
MRTTLAIDDDVLIAAKAMAAQQHRSLGAVISELARRSLRRPRSGGERNGIPLLSTQPDAPPVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT105680 NZ_CP029452:c912480-912253 [Sinorhizobium fredii CCBAU 25509]
ATGAGAACGACACTTGCGATCGATGACGATGTCCTGATCGCCGCCAAGGCAATGGCGGCCCAGCAGCACCGCAGTCTCGG
CGCGGTCATTTCCGAGCTGGCGAGGCGTTCTTTGCGCCGGCCACGCAGTGGTGGCGAGCGCAATGGAATTCCTCTGCTGT
CGACGCAACCCGATGCCCCGCCAGTTACCCTCGAAATCGTCAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A2Z3GJ97

Antitoxin

• AlphaFold DB: A0A2Z3GA74