Detailed information of TA system TA105679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 846002..846931
• Replicon: plasmid pSF25509b
• Accession: NZ_CP029452
• Organism: Sinorhizobium fredii CCBAU 25509

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2Z3GD60
• Locus tag: AOX55_RS24825
• Protein ID: WP_037396246.1
• Coordinates: 846377..846931 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2Z3GB78
• Locus tag: AOX55_RS24820
• Protein ID: WP_037396248.1
• Coordinates: 846002..846373 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX55_RS24800	841872..842255	+	384	WP_037396259.1	RidA family protein	-
AOX55_RS24805	842271..844259	+	1989	WP_037396257.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
AOX55_RS24810	844256..845125	+	870	WP_037396254.1	hydroxymethylglutaryl-CoA lyase	-
AOX55_RS24815	845122..845907	+	786	WP_037396251.1	crotonase/enoyl-CoA hydratase family protein	-
AOX55_RS24820	846002..846373	+	372	WP_037396248.1	DUF2384 domain-containing protein	Antitoxin
AOX55_RS24825	846377..846931	+	555	WP_037396246.1	RES domain-containing protein	Toxin
AOX55_RS24830	846941..848305	-	1365	WP_037396244.1	FAD-binding oxidoreductase	-
AOX55_RS24835	848451..849557	-	1107	WP_109982244.1	sulfate/molybdate ABC transporter ATP-binding protein	-
AOX55_RS24840	849573..850460	-	888	WP_037396228.1	sulfate ABC transporter permease subunit CysW	-
AOX55_RS24845	850450..851307	-	858	WP_037396225.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / katA	1..2210745	2210745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-173)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20769.55 Da
• Isoelectric Point: 5.2816

>T105679 WP_037396246.1 NZ_CP029452:846377-846931 [Sinorhizobium fredii CCBAU 25509]
MKKNISDPSASRSDLDVATPAEPMTLWRAFVPRWAHMPLSGEGAARFGGRWNPVGMPTIYAARELSTAWAEYNQGFVQHP
ALIVQLELCDARLADLTDGDVLAELGLEDTIHRCEWRDELDKSLVPQTHRIQADLWKRGYDGVIYPSYMSPGGTCVALWR
WRDRPRLDVIDPEGRLPNSPASWL

Nucleotide

• Length: 555 bp

>T105679 NZ_CP029452:846377-846931 [Sinorhizobium fredii CCBAU 25509]
ATGAAGAAAAATATTAGCGATCCGTCCGCCTCCCGCTCCGACCTGGACGTGGCGACGCCGGCCGAGCCGATGACGCTCTG
GCGCGCTTTCGTGCCGCGCTGGGCCCATATGCCGCTTTCCGGCGAGGGCGCGGCTCGCTTCGGGGGCCGATGGAATCCGG
TCGGCATGCCGACGATCTACGCCGCGCGTGAACTTTCCACCGCCTGGGCGGAATATAATCAGGGCTTCGTCCAGCATCCG
GCGCTGATCGTGCAGCTCGAGCTTTGCGACGCGAGGCTGGCAGATCTCACGGATGGCGATGTCCTTGCCGAACTCGGGCT
CGAAGATACGATTCACCGCTGCGAGTGGCGCGACGAACTCGACAAGAGCCTGGTGCCGCAGACGCACAGGATCCAGGCCG
ACCTTTGGAAGCGCGGATACGATGGCGTGATCTACCCGTCCTACATGTCGCCCGGCGGCACCTGTGTCGCCCTATGGCGC
TGGCGTGACCGCCCACGGCTCGACGTCATCGACCCGGAGGGCCGGCTGCCGAATTCGCCGGCGTCGTGGCTTTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13167.29 Da
• Isoelectric Point: 9.5098

>AT105679 WP_037396248.1 NZ_CP029452:846002-846373 [Sinorhizobium fredii CCBAU 25509]
MVAVDFQIPAARFGDDHSPFLSARLVADRLGITLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAYDLVGEDKADKVLAYLEAIRAGVYA

Nucleotide

• Length: 372 bp

>AT105679 NZ_CP029452:846002-846373 [Sinorhizobium fredii CCBAU 25509]
ATGGTGGCGGTCGATTTTCAAATACCAGCGGCGCGTTTCGGGGATGATCATTCCCCATTCCTGTCGGCACGTCTCGTCGC
CGACCGGCTCGGCATCACGCTCGCCGAACTCGCCAAACTGATTGGAGTCGCGCGCAACACGCTGACGGCAAAGTCCGGCG
CGCGAAAGGTCGACAGCGCCTTGAGCAAGGTCGTTCGCATCCTCGCCATGGCGTCCGAGATGGCCGGCGACGAGGCGCGC
GCCGTCATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCATATGATCTGGTCGGCGAGGACAAGGC
CGACAAGGTTCTCGCCTATCTCGAGGCGATCCGCGCCGGCGTCTACGCCTAG

Structures

Toxin

• AlphaFold DB: A0A2Z3GD60

Antitoxin

• AlphaFold DB: A0A2Z3GB78