Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 214683..215359 Replicon plasmid pSF25509b
Accession NZ_CP029452
Organism Sinorhizobium fredii CCBAU 25509

Toxin (Protein)


Gene name vapC Uniprot ID A0A2Z3G3H6
Locus tag AOX55_RS21510 Protein ID WP_037395553.1
Coordinates 214934..215359 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A249Q515
Locus tag AOX55_RS21505 Protein ID WP_037395557.1
Coordinates 214683..214937 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOX55_RS21485 210068..211070 - 1003 Protein_203 alpha/beta hydrolase -
AOX55_RS21490 211134..211931 - 798 WP_037395561.1 alpha/beta hydrolase -
AOX55_RS21495 212056..212991 + 936 WP_014332647.1 LysR family transcriptional regulator -
AOX55_RS21500 213150..214115 - 966 WP_109982204.1 MBL fold metallo-hydrolase -
AOX55_RS21505 214683..214937 + 255 WP_037395557.1 plasmid stabilization protein Antitoxin
AOX55_RS21510 214934..215359 + 426 WP_037395553.1 type II toxin-antitoxin system VapC family toxin Toxin
AOX55_RS21515 215899..216540 - 642 WP_037395551.1 AhpC/TSA family protein -
AOX55_RS21520 216925..217458 - 534 WP_037395549.1 GXWXG domain-containing protein -
AOX55_RS21525 217573..217773 - 201 WP_037395547.1 hypothetical protein -
AOX55_RS21535 218510..219115 - 606 WP_037395543.1 hypothetical protein -
AOX55_RS21540 219152..219466 - 315 WP_037395542.1 PepSY domain-containing protein -
AOX55_RS33725 219559..219903 + 345 WP_153451912.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB / katA 1..2210745 2210745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15157.46 Da        Isoelectric Point: 5.7185

>T105677 WP_037395553.1 NZ_CP029452:214934-215359 [Sinorhizobium fredii CCBAU 25509]
MIVLDTKVVSEAMEPFPNPVVHSWLNEQVAETLYLSSVTLAELMFGIGALPDGRRKKALSETLDGVLELFGDRVLPFDNG
AARHYAALAMKARAAGKGFPTPDGYIAAIAASKGFIIATRDVSPFEAAGLTVVNPWNNQRI

Download         Length: 426 bp

>T105677 NZ_CP029452:214934-215359 [Sinorhizobium fredii CCBAU 25509]
ATGATCGTCTTGGATACGAAGGTCGTCTCCGAGGCGATGGAACCGTTTCCCAATCCGGTCGTGCACTCCTGGCTGAACGA
GCAGGTCGCCGAGACCCTATATCTGTCCAGTGTGACGTTGGCCGAACTGATGTTTGGAATCGGTGCATTGCCGGATGGCC
GGCGCAAGAAAGCTCTGAGCGAAACGCTTGACGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACAACGGA
GCTGCCCGGCATTATGCCGCCTTAGCCATGAAGGCCCGAGCGGCCGGCAAGGGCTTCCCGACGCCCGATGGATATATTGC
CGCCATTGCCGCCTCCAAGGGATTTATCATAGCGACGCGTGATGTAAGCCCCTTTGAAGCGGCAGGACTTACCGTCGTAA
ATCCTTGGAATAATCAACGAATATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9512.92 Da        Isoelectric Point: 7.2068

>AT105677 WP_037395557.1 NZ_CP029452:214683-214937 [Sinorhizobium fredii CCBAU 25509]
MAILTVRNVPDEVHRALRVRAAMHGRSTEAKVRDILESVVKPEQRVRMGDALAEVGRRLGLTNDDFAVFDEVRDKAPAEP
MRFE

Download         Length: 255 bp

>AT105677 NZ_CP029452:214683-214937 [Sinorhizobium fredii CCBAU 25509]
ATGGCCATCCTGACAGTGAGGAATGTGCCCGATGAGGTGCATCGTGCTTTACGCGTGCGGGCGGCGATGCACGGCCGCAG
CACCGAGGCGAAAGTCCGTGACATTCTGGAAAGTGTGGTAAAGCCCGAGCAGCGCGTTCGCATGGGGGATGCCTTGGCTG
AGGTCGGCCGGCGGCTGGGACTGACCAATGATGATTTCGCAGTGTTTGATGAGGTACGTGACAAGGCGCCGGCGGAGCCA
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3G3H6

Antitoxin

Source ID Structure
AlphaFold DB A0A249Q515

References