Detailed information of TA system TA105676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 139763..140331
• Replicon: chromosome
• Accession: NZ_CP029451
• Organism: Sinorhizobium fredii CCBAU 25509

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: G9AA43
• Locus tag: AOX55_RS00695
• Protein ID: WP_014327160.1
• Coordinates: 140086..140331 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: G9AA42
• Locus tag: AOX55_RS00690
• Protein ID: WP_014327159.1
• Coordinates: 139763..140089 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX55_RS00660	134900..135217	+	318	WP_014327153.1	hypothetical protein	-
AOX55_RS33455	135217..135384	+	168	WP_162129941.1	hypothetical protein	-
AOX55_RS00665	135385..135948	+	564	WP_050983983.1	hypothetical protein	-
AOX55_RS00670	135945..136889	+	945	WP_037391847.1	toprim domain-containing protein	-
AOX55_RS00675	136886..137959	+	1074	WP_014327155.1	hypothetical protein	-
AOX55_RS00680	138046..138594	+	549	WP_037391910.1	ribonuclease HI	-
AOX55_RS00685	138650..139168	+	519	WP_014327157.1	hypothetical protein	-
AOX55_RS00690	139763..140089	-	327	WP_014327159.1	BrnA antitoxin family protein	Antitoxin
AOX55_RS00695	140086..140331	-	246	WP_014327160.1	BrnT family toxin	Toxin
AOX55_RS33460	140445..140663	+	219	WP_148280389.1	hypothetical protein	-
AOX55_RS33465	140653..141162	+	510	WP_157784626.1	terminase small subunit protein	-
AOX55_RS00705	141065..142483	+	1419	WP_050983985.1	terminase large subunit	-
AOX55_RS00710	142483..144540	+	2058	WP_037391850.1	phage portal protein	-
AOX55_RS00715	144540..144776	+	237	WP_037391852.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	131487..180142	48655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9123.63 Da
• Isoelectric Point: 9.6920

>T105676 WP_014327160.1 NZ_CP029451:c140331-140086 [Sinorhizobium fredii CCBAU 25509]
MKITYDENKRLTNIEKHGYDFAELDMEFFASSVVVPAKEGRFMAIGVLRNGVVAVVFARLGKEGISIISMRDASRKERSL
L

Nucleotide

• Length: 246 bp

>T105676 NZ_CP029451:c140331-140086 [Sinorhizobium fredii CCBAU 25509]
ATGAAAATCACCTACGACGAAAACAAGCGCCTAACCAACATCGAAAAGCACGGCTATGATTTTGCCGAGCTGGACATGGA
GTTCTTCGCTTCGTCCGTGGTGGTTCCGGCCAAGGAAGGCCGCTTCATGGCAATCGGCGTTCTGCGCAATGGCGTTGTCG
CCGTCGTCTTCGCCCGCCTTGGCAAGGAAGGCATCTCGATTATCTCTATGCGTGACGCAAGCCGGAAGGAAAGGAGCTTA
CTATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12204.80 Da
• Isoelectric Point: 10.0528

>AT105676 WP_014327159.1 NZ_CP029451:c140089-139763 [Sinorhizobium fredii CCBAU 25509]
MTNLSRRIRQISDKQEAEIQRKIASDPDAPEATDEQLGQAKPFAEAFPDLAESIKRARGRPAVEHPRQQISIRLDPDVIE
HYKKNCGKGWQSRMNEDLRKAAGLKKAG

Nucleotide

• Length: 327 bp

>AT105676 NZ_CP029451:c140089-139763 [Sinorhizobium fredii CCBAU 25509]
ATGACGAACCTATCCCGCCGCATCCGCCAAATCTCCGACAAGCAGGAGGCTGAAATCCAACGCAAGATAGCCAGTGATCC
GGACGCCCCGGAGGCTACAGACGAGCAGCTTGGGCAGGCCAAGCCATTCGCCGAGGCATTCCCTGACTTGGCCGAGAGCA
TCAAGCGCGCGAGGGGCCGGCCGGCCGTTGAGCACCCGCGGCAGCAGATATCCATCCGCTTAGACCCGGATGTCATTGAG
CACTACAAGAAGAACTGCGGCAAGGGCTGGCAGAGCCGAATGAATGAGGACCTTAGAAAGGCCGCCGGCCTGAAGAAAGC
CGGCTGA

Structures

Toxin

• AlphaFold DB: G9AA43

Antitoxin

• AlphaFold DB: G9AA42