Detailed information of TA system TA105675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 131487..132135
• Replicon: chromosome
• Accession: NZ_CP029451
• Organism: Sinorhizobium fredii CCBAU 25509

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G9AA33
• Locus tag: AOX55_RS00640
• Protein ID: WP_014327150.1
• Coordinates: 131881..132135 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AOX55_RS00635
• Protein ID: WP_037391843.1
• Coordinates: 131487..131867 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOX55_RS00620	129203..129868	+	666	WP_014327146.1	CDP-alcohol phosphatidyltransferase family protein	-
AOX55_RS00625	129865..130476	+	612	WP_014327147.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
AOX55_RS00630	130484..131425	+	942	WP_037391841.1	phosphatidate cytidylyltransferase	-
AOX55_RS00635	131487..131867	-	381	WP_037391843.1	helix-turn-helix transcriptional regulator	Antitoxin
AOX55_RS00640	131881..132135	-	255	WP_014327150.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOX55_RS00645	132573..133865	+	1293	WP_014327151.1	site-specific integrase	-
AOX55_RS00650	133862..134431	-	570	WP_127624429.1	hypothetical protein	-
AOX55_RS00655	134571..134783	+	213	WP_014327152.1	hypothetical protein	-
AOX55_RS00660	134900..135217	+	318	WP_014327153.1	hypothetical protein	-
AOX55_RS33455	135217..135384	+	168	WP_162129941.1	hypothetical protein	-
AOX55_RS00665	135385..135948	+	564	WP_050983983.1	hypothetical protein	-
AOX55_RS00670	135945..136889	+	945	WP_037391847.1	toprim domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	131487..180142	48655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-125)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9407.94 Da
• Isoelectric Point: 10.8582

>T105675 WP_014327150.1 NZ_CP029451:c132135-131881 [Sinorhizobium fredii CCBAU 25509]
MKTIRYTREALKTLTQIPANVAKRIRSKIEQYASDPASLANNIKALKGLQGTHRLRVGDWRVIFTEDGEIIAIKKIAPRG
GAYD

Nucleotide

• Length: 255 bp

>T105675 NZ_CP029451:c132135-131881 [Sinorhizobium fredii CCBAU 25509]
ATGAAAACGATCCGGTACACCCGTGAAGCGCTGAAAACCTTGACACAAATTCCCGCGAACGTTGCCAAGCGGATCCGCTC
GAAAATCGAGCAATATGCATCGGACCCGGCAAGTCTCGCTAACAACATCAAGGCACTGAAAGGGTTGCAAGGTACACATC
GGCTTCGGGTCGGAGACTGGCGCGTTATCTTTACGGAAGACGGCGAAATCATCGCCATCAAGAAGATCGCCCCGCGCGGC
GGCGCATACGACTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14006.05 Da
• Isoelectric Point: 4.4452

>AT105675 WP_037391843.1 NZ_CP029451:c131867-131487 [Sinorhizobium fredii CCBAU 25509]
MVLQFITTPAGDRMVVMAEDEYKMLLHAAEAAEDAEDVADVKRINERLARGEEELIPADIVNRILDGESKVKVWREYRKL
TLRELAAKVDISPAYLSQIEGGSRDGTFETMKKIAAALGITVDELA

Nucleotide

• Length: 381 bp

>AT105675 NZ_CP029451:c131867-131487 [Sinorhizobium fredii CCBAU 25509]
ATGGTACTACAGTTCATCACTACCCCGGCCGGCGACCGAATGGTCGTCATGGCCGAGGACGAATACAAGATGCTTTTGCA
TGCCGCAGAGGCTGCCGAGGACGCCGAAGACGTCGCGGACGTAAAGCGGATCAATGAGCGCCTAGCTCGCGGCGAAGAAG
AGTTGATCCCCGCCGATATCGTCAACCGCATCCTTGATGGCGAAAGCAAGGTGAAGGTCTGGCGCGAGTACAGGAAACTA
ACCCTCCGAGAACTCGCGGCCAAGGTCGATATCAGCCCGGCGTACCTTTCTCAAATCGAGGGCGGCAGCCGCGACGGCAC
CTTTGAGACGATGAAGAAGATAGCGGCCGCTCTCGGTATCACGGTGGACGAACTCGCATAA

Structures

Toxin

• AlphaFold DB: G9AA33