Detailed information of TA system TA105663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 995465..996012
• Replicon: chromosome
• Accession: NZ_CP029449
• Organism: Serratia marcescens strain CAV1761

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: DKC05_RS06735
• Protein ID: WP_047728145.1
• Coordinates: 995465..995773 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0G3SUU9
• Locus tag: DKC05_RS06740
• Protein ID: WP_033633717.1
• Coordinates: 995776..996012 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKC05_RS06715	991233..992390	-	1158	WP_047728141.1	YbfB/YjiJ family MFS transporter	-
DKC05_RS06720	992496..993416	+	921	WP_038880964.1	LysR family transcriptional regulator	-
DKC05_RS06725	993447..994571	+	1125	WP_038880962.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
DKC05_RS06730	994586..995428	+	843	WP_047728144.1	S-formylglutathione hydrolase	-
DKC05_RS06735	995465..995773	-	309	WP_047728145.1	CcdB family protein	Toxin
DKC05_RS06740	995776..996012	-	237	WP_033633717.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DKC05_RS06745	996100..997008	-	909	WP_195826229.1	glutathione ABC transporter permease GsiD	-
DKC05_RS06750	997018..997938	-	921	WP_038880960.1	glutathione ABC transporter permease GsiC	-
DKC05_RS06755	997991..999526	-	1536	WP_047728148.1	glutathione ABC transporter substrate-binding protein GsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11472.26 Da
• Isoelectric Point: 5.0196

>T105663 WP_047728145.1 NZ_CP029449:c995773-995465 [Serratia marcescens]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLVIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T105663 NZ_CP029449:c995773-995465 [Serratia marcescens]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAGTGATCCCTCTTTATGCTTTGGATAACGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AAATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCTATCGATTTTCTGATTGATGGCTTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8994.07 Da
• Isoelectric Point: 5.0807

>AT105663 WP_033633717.1 NZ_CP029449:c996012-995776 [Serratia marcescens]
MANNNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT105663 NZ_CP029449:c996012-995776 [Serratia marcescens]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCCGTATTGACGGACGCATTGAGCGTCCGCGTCAGGGATATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGCGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SUU9