Detailed information of TA system TA105653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 200560..201299
• Replicon: plasmid pCAV1761-205
• Accession: NZ_CP029448
• Organism: Serratia marcescens strain CAV1761

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DKC05_RS01940
• Protein ID: WP_071845235.1
• Coordinates: 200814..201299 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: DKC05_RS01935
• Protein ID: WP_012540086.1
• Coordinates: 200560..200826 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKC05_RS01905	195791..196327	-	537	WP_013087166.1	GNAT family N-acetyltransferase	-
DKC05_RS01910	196452..198203	-	1752	WP_162875198.1	arsenical pump-driving ATPase	-
DKC05_RS01915	198230..198592	-	363	WP_047727557.1	arsenic metallochaperone ArsD family protein	-
DKC05_RS01920	198668..199213	-	546	WP_012540227.1	sigma-70 family RNA polymerase sigma factor	-
DKC05_RS01925	199222..199935	-	714	WP_047727595.1	arsenical resistance protein ArsH	-
DKC05_RS01930	199937..200260	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
DKC05_RS01935	200560..200826	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
DKC05_RS01940	200814..201299	+	486	WP_071845235.1	GNAT family N-acetyltransferase	Toxin
DKC05_RS01945	201640..201798	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
DKC05_RS01950	202410..202628	+	219	WP_009651962.1	hypothetical protein	-
DKC05_RS01955	202649..202972	+	324	WP_009651959.1	hypothetical protein	-
DKC05_RS01960	203025..203372	+	348	WP_009651960.1	hypothetical protein	-
DKC05_RS01965	203466..203612	+	147	WP_047727556.1	hypothetical protein	-
DKC05_RS01970	203662..204495	+	834	WP_009651958.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-30	-	1..204825	204825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17766.57 Da
• Isoelectric Point: 9.8560

>T105653 WP_071845235.1 NZ_CP029448:200814-201299 [Serratia marcescens]
VGRVTAPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T105653 NZ_CP029448:200814-201299 [Serratia marcescens]
GTGGGACGTGTAACAGCACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTTGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTTCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT105653 WP_012540086.1 NZ_CP029448:200560-200826 [Serratia marcescens]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT105653 NZ_CP029448:200560-200826 [Serratia marcescens]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCCAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCCGAGAATGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGACCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AWX8