Detailed information of TA system TA105651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5130736..5131276
• Replicon: chromosome
• Accession: NZ_CP029427
• Organism: Bradyrhizobium symbiodeficiens strain 85S1MB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CIT39_RS24335
• Protein ID: WP_094977258.1
• Coordinates: 5131016..5131276 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CIT39_RS24330
• Protein ID: WP_094977259.1
• Coordinates: 5130736..5131029 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIT39_RS24300	5126483..5126896	-	414	WP_094977261.1	rhodanese-like domain-containing protein	-
CIT39_RS24305	5127030..5128205	-	1176	WP_094977260.1	VWA domain-containing protein	-
CIT39_RS24310	5128329..5128574	+	246	WP_018318587.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
CIT39_RS24315	5128670..5129512	+	843	WP_027529387.1	MoxR family ATPase	-
CIT39_RS33760	5130329..5130736	-	408	WP_155526033.1	hypothetical protein	-
CIT39_RS24330	5130736..5131029	-	294	WP_094977259.1	BrnA antitoxin family protein	Antitoxin
CIT39_RS24335	5131016..5131276	-	261	WP_094977258.1	BrnT family toxin	Toxin
CIT39_RS24340	5131460..5133106	-	1647	WP_094977257.1	GMC family oxidoreductase N-terminal domain-containing protein	-
CIT39_RS24345	5133124..5133717	-	594	WP_094977256.1	GNAT family N-acetyltransferase	-
CIT39_RS24350	5133877..5134803	-	927	WP_094977255.1	SMP-30/gluconolactonase/LRE family protein	-
CIT39_RS24355	5135052..5136155	+	1104	WP_094977254.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9864.46 Da
• Isoelectric Point: 10.5508

>T105651 WP_094977258.1 NZ_CP029427:c5131276-5131016 [Bradyrhizobium symbiodeficiens]
MKIVWDEPKRLANLDKHELDFADLNETFFDNALVIPSHNKSKRWVAIGVSIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T105651 NZ_CP029427:c5131276-5131016 [Bradyrhizobium symbiodeficiens]
ATGAAAATCGTCTGGGATGAACCGAAGCGGCTCGCCAACCTCGACAAGCACGAGCTGGATTTTGCCGATCTCAACGAGAC
GTTCTTCGATAACGCACTGGTCATTCCGTCGCACAACAAGAGCAAACGCTGGGTTGCGATCGGCGTGAGCATCCGTGGTG
TCATCGTTGTCGTGTTCGCCCGGCTGGGACGCGAAGGCGTCAGCATCATCAGCATGCGACCGGCAAGCCGCAGCGAAAGG
AAACTCTATGCCGAACGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.42 Da
• Isoelectric Point: 11.3548

>AT105651 WP_094977259.1 NZ_CP029427:c5131029-5130736 [Bradyrhizobium symbiodeficiens]
MPNAKKKSGYTKRDLRAVSDTPELTRADFAKARPFSEVFPDLSAAIRKGRGPNKAPTKTLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT105651 NZ_CP029427:c5131029-5130736 [Bradyrhizobium symbiodeficiens]
ATGCCGAACGCTAAAAAGAAGTCGGGTTACACCAAGAGAGACCTGCGTGCGGTCAGCGACACGCCGGAGCTGACAAGGGC
CGATTTCGCCAAGGCGCGGCCTTTCTCTGAAGTCTTCCCTGATCTCAGCGCAGCCATCCGCAAGGGCCGAGGTCCGAACA
AGGCGCCGACAAAGACGCTCGTCTCCCTGCGCCTCAGCCCAGAGGTGGTCGAGCATTTCAAATCGACCGGACCGGGCTGG
CAGTCGCGGATCGACGAAACCTTGCGCAAGGCGGTGAAGCGCAAGGCGTCGTGA