Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-Phd
Location 6799445..6800112 Replicon chromosome
Accession NZ_CP029425
Organism Bradyrhizobium ottawaense strain OO99

Toxin (Protein)


Gene name vapC Uniprot ID A0A2U8PF08
Locus tag CIT37_RS32685 Protein ID WP_028144859.1
Coordinates 6799702..6800112 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2U8PFU4
Locus tag CIT37_RS32680 Protein ID WP_028144858.1
Coordinates 6799445..6799702 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIT37_RS32655 6794844..6795284 + 441 WP_018323001.1 response regulator -
CIT37_RS32660 6795281..6796384 + 1104 WP_038970183.1 response regulator -
CIT37_RS32665 6796448..6797128 + 681 WP_028144855.1 leucyl/phenylalanyl-tRNA--protein transferase -
CIT37_RS32670 6797169..6798203 - 1035 WP_028144856.1 DUF2155 domain-containing protein -
CIT37_RS32675 6798410..6799357 + 948 WP_028144857.1 alpha/beta hydrolase -
CIT37_RS32680 6799445..6799702 + 258 WP_028144858.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CIT37_RS32685 6799702..6800112 + 411 WP_028144859.1 type II toxin-antitoxin system VapC family toxin Toxin
CIT37_RS32690 6800150..6801148 + 999 WP_095424248.1 Na+-dependent transporter -
CIT37_RS32695 6801145..6802083 - 939 WP_038970181.1 GlxA family transcriptional regulator -
CIT37_RS32700 6802180..6802866 + 687 WP_038970189.1 DJ-1/PfpI family protein -
CIT37_RS32705 6803368..6803775 - 408 WP_028144863.1 NADH:ubiquinone oxidoreductase subunit NDUFA12 -
CIT37_RS32710 6804019..6804690 + 672 WP_095424249.1 BA14K family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-118)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(4-53)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15059.18 Da        Isoelectric Point: 5.1957

>T105649 WP_028144859.1 NZ_CP029425:6799702-6800112 [Bradyrhizobium ottawaense]
MFLLDTVTLSELRRTRRNPSVVAWMERQRTADLFLSVVSIGEIERGIERQRAADPSFADALATWLDRVLALYGTQVLPFD
LQTARRWGALSAALGNDSADLMIAATALEHGLTVVTRNVSDFEPTGVRVVNPFEGK

Download         Length: 411 bp

>T105649 NZ_CP029425:6799702-6800112 [Bradyrhizobium ottawaense]
ATGTTTCTGCTCGACACCGTCACGCTGTCCGAGCTGCGTCGGACCAGACGCAACCCGTCGGTGGTCGCCTGGATGGAGCG
GCAGCGAACGGCAGATCTTTTCCTCAGCGTCGTCAGCATCGGCGAAATCGAACGCGGAATCGAACGTCAGCGCGCTGCAG
ATCCGAGCTTTGCCGATGCGCTCGCCACCTGGCTGGATCGCGTTCTCGCTCTCTACGGCACACAGGTCTTGCCGTTTGAC
CTCCAGACCGCGCGTCGCTGGGGAGCTCTCAGCGCCGCGCTCGGCAATGACAGCGCAGATCTCATGATTGCCGCCACCGC
GCTCGAGCATGGACTTACTGTCGTGACGCGCAATGTCTCGGACTTTGAGCCTACGGGAGTTCGCGTCGTAAATCCATTTG
AAGGCAAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9619.96 Da        Isoelectric Point: 8.9339

>AT105649 WP_028144858.1 NZ_CP029425:6799445-6799702 [Bradyrhizobium ottawaense]
MTKRVWSVQDAKNRFSEVVHAAQRAPQTVTRHGKPAVVILNAAEFERLQELQRARAPSLTDLLLEMPQDDGEFPRARVAD
RDIGL

Download         Length: 258 bp

>AT105649 NZ_CP029425:6799445-6799702 [Bradyrhizobium ottawaense]
ATGACCAAGCGAGTCTGGTCGGTGCAGGATGCAAAAAACCGCTTCAGCGAGGTGGTCCATGCGGCGCAGCGCGCACCGCA
AACGGTGACGCGGCATGGCAAACCCGCTGTGGTGATCTTGAATGCCGCAGAGTTTGAACGCTTGCAGGAGCTGCAACGCG
CTCGGGCGCCCTCTCTGACCGATCTGCTTCTTGAAATGCCGCAAGACGACGGGGAATTTCCGCGCGCGCGTGTCGCGGAT
CGTGACATCGGCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U8PF08

Antitoxin

Source ID Structure
AlphaFold DB A0A2U8PFU4

References