Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2581066..2581724 Replicon chromosome
Accession NZ_CP029425
Organism Bradyrhizobium ottawaense strain OO99

Toxin (Protein)


Gene name vapC Uniprot ID A0A2U8P577
Locus tag CIT37_RS12155 Protein ID WP_028139223.1
Coordinates 2581066..2581470 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2U8PJT6
Locus tag CIT37_RS12160 Protein ID WP_038949867.1
Coordinates 2581470..2581724 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIT37_RS12140 2578399..2579121 - 723 WP_028139226.1 ABC transporter ATP-binding protein -
CIT37_RS12145 2579177..2579821 + 645 WP_038949866.1 arylesterase -
CIT37_RS12150 2579996..2580532 + 537 WP_028139224.1 RNA 2',3'-cyclic phosphodiesterase -
CIT37_RS12155 2581066..2581470 - 405 WP_028139223.1 type II toxin-antitoxin system VapC family toxin Toxin
CIT37_RS12160 2581470..2581724 - 255 WP_038949867.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CIT37_RS12165 2581908..2583092 + 1185 WP_028139221.1 cell division protein ZapE -
CIT37_RS12170 2583354..2584322 + 969 WP_008145224.1 malate dehydrogenase -
CIT37_RS12175 2584529..2585725 + 1197 WP_038949870.1 ADP-forming succinate--CoA ligase subunit beta -
CIT37_RS12180 2585730..2586209 + 480 WP_038949871.1 DUF1579 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14613.69 Da        Isoelectric Point: 4.5209

>T105648 WP_028139223.1 NZ_CP029425:c2581470-2581066 [Bradyrhizobium ottawaense]
MVIDTSAIVAIALDEDDAADMERLIVDDPIRLISAATVLEATMVIETRLGDAGGREFDLWLVKIGAEIVAVDAVQADAAR
RAWRRFGKGRHAASLNYGDCFSYALAMTRGEPLLFKGEDFAKTDINRSSVTPLR

Download         Length: 405 bp

>T105648 NZ_CP029425:c2581470-2581066 [Bradyrhizobium ottawaense]
ATGGTGATCGACACATCCGCGATCGTGGCAATCGCGCTCGACGAAGACGATGCCGCGGATATGGAGCGACTGATCGTGGA
CGATCCGATCCGCCTGATTTCCGCGGCGACGGTGCTTGAAGCGACGATGGTGATCGAGACGCGGCTGGGCGATGCGGGCG
GGCGCGAGTTCGATCTCTGGCTCGTCAAGATCGGCGCTGAGATCGTCGCGGTCGATGCCGTGCAAGCCGACGCGGCCCGG
CGCGCCTGGCGGCGTTTCGGAAAAGGGCGTCACGCCGCGTCGCTCAATTACGGTGACTGCTTTTCTTATGCGCTGGCCAT
GACCCGTGGCGAACCACTGCTGTTCAAAGGCGAGGACTTTGCGAAGACCGACATCAACCGGTCGAGCGTGACGCCGCTGC
GATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9792.08 Da        Isoelectric Point: 5.2171

>AT105648 WP_038949867.1 NZ_CP029425:c2581724-2581470 [Bradyrhizobium ottawaense]
MALSIKDPETEQLARSLAQLTGENITTATKRAIEERLRRLNAQTHKDALLEDLAEIRRRWSEMRVLDDRKPEEILGYDDY
GLPR

Download         Length: 255 bp

>AT105648 NZ_CP029425:c2581724-2581470 [Bradyrhizobium ottawaense]
ATGGCGCTGAGCATCAAGGACCCCGAAACCGAGCAACTGGCCCGGAGTCTGGCACAATTGACGGGGGAGAACATCACGAC
TGCGACCAAGCGCGCGATCGAGGAGCGATTGCGCCGGCTCAACGCTCAAACACACAAGGACGCGCTGCTTGAAGACCTTG
CCGAGATCAGACGGCGCTGGAGCGAGATGCGCGTCCTGGACGACCGCAAGCCTGAAGAAATCCTCGGATACGACGATTAT
GGATTGCCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U8P577

Antitoxin

Source ID Structure
AlphaFold DB A0A2U8PJT6

References