Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 25670..26271 Replicon plasmid unnamed2
Accession NZ_CP029422
Organism Escherichia coli strain 3385

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag DFS94_RS25090 Protein ID WP_001216034.1
Coordinates 25670..26050 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DFS94_RS25095 Protein ID WP_001190712.1
Coordinates 26050..26271 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DFS94_RS25055 20721..21653 - 933 WP_000081352.1 homocysteine S-methyltransferase -
DFS94_RS25060 21640..23043 - 1404 WP_001373486.1 S-methylmethionine permease -
DFS94_RS25065 23287..24267 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
DFS94_RS25070 24495..24812 + 318 WP_001513659.1 hypothetical protein -
DFS94_RS25075 24762..25175 - 414 Protein_29 DDE-type integrase/transposase/recombinase -
DFS94_RS25080 25099..25458 - 360 WP_001513660.1 hypothetical protein -
DFS94_RS25085 25486..25665 - 180 WP_001513661.1 hypothetical protein -
DFS94_RS25090 25670..26050 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DFS94_RS25095 26050..26271 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DFS94_RS25100 26454..28010 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
DFS94_RS25105 28007..29278 + 1272 WP_023142242.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..89323 89323


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T105633 WP_001216034.1 NZ_CP029422:c26050-25670 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T105633 NZ_CP029422:c26050-25670 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT105633 WP_001190712.1 NZ_CP029422:c26271-26050 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT105633 NZ_CP029422:c26271-26050 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References