Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3559726..3560223 Replicon chromosome
Accession NZ_CP029420
Organism Escherichia coli strain 3385

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DFS94_RS17580 Protein ID WP_001173343.1
Coordinates 3560050..3560223 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag DFS94_RS17575 Protein ID WP_001312331.1
Coordinates 3559726..3560022 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DFS94_RS17565 3555083..3559012 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
DFS94_RS17570 3559226..3559498 + 273 WP_000405647.1 N-acetyltransferase -
DFS94_RS17575 3559726..3560022 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DFS94_RS17580 3560050..3560223 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DFS94_RS17585 3560342..3561859 - 1518 WP_000003806.1 lysine--tRNA ligase -
DFS94_RS17590 3562096..3563553 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3544136..3569077 24941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T105624 WP_001173343.1 NZ_CP029420:3560050-3560223 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T105624 NZ_CP029420:3560050-3560223 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT105624 WP_001312331.1 NZ_CP029420:3559726-3560022 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT105624 NZ_CP029420:3559726-3560022 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References