Detailed information of TA system TA105622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2744973..2745773
• Replicon: chromosome
• Accession: NZ_CP029420
• Organism: Escherichia coli strain 3385

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: DFS94_RS13785
• Protein ID: WP_000342452.1
• Coordinates: 2744973..2745500 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: DFS94_RS13790
• Protein ID: WP_001277107.1
• Coordinates: 2745507..2745773 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFS94_RS13760	2740049..2740816	-	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DFS94_RS13765	2740813..2742090	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
DFS94_RS13770	2742087..2743013	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DFS94_RS13775	2743061..2744170	-	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
DFS94_RS13780	2744593..2744976	+	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
DFS94_RS13785	2744973..2745500	-	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
DFS94_RS13790	2745507..2745773	-	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
DFS94_RS13795	2745922..2747025	-	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
DFS94_RS13800	2747296..2748150	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
DFS94_RS13805	2748395..2749453	-	1059	WP_001042013.1	permease-like cell division protein FtsX	-
DFS94_RS13810	2749446..2750114	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T105622 WP_000342452.1 NZ_CP029420:c2745500-2744973 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T105622 NZ_CP029420:c2745500-2744973 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT105622 WP_001277107.1 NZ_CP029420:c2745773-2745507 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT105622 NZ_CP029420:c2745773-2745507 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57