Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1236542..1237373 Replicon chromosome
Accession NZ_CP029420
Organism Escherichia coli strain 3385

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag DFS94_RS06610 Protein ID WP_000854815.1
Coordinates 1236999..1237373 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag DFS94_RS06605 Protein ID WP_001280918.1
Coordinates 1236542..1236910 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DFS94_RS06565 1232865..1233236 + 372 WP_138976321.1 hypothetical protein -
DFS94_RS06575 1233457..1234275 + 819 WP_001542275.1 DUF945 domain-containing protein -
DFS94_RS06580 1234275..1234520 + 246 WP_001164966.1 hypothetical protein -
DFS94_RS06585 1234614..1235087 + 474 WP_001542276.1 antirestriction protein -
DFS94_RS06590 1235103..1235579 + 477 WP_001186200.1 RadC family protein -
DFS94_RS06595 1235642..1235863 + 222 WP_000692345.1 DUF987 domain-containing protein -
DFS94_RS06600 1235882..1236526 + 645 WP_000086752.1 hypothetical protein -
DFS94_RS06605 1236542..1236910 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
DFS94_RS06610 1236999..1237373 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
DFS94_RS06615 1237370..1237564 + 195 WP_000988600.1 hypothetical protein -
DFS94_RS06620 1237577..1237690 + 114 WP_001161660.1 DUF957 domain-containing protein -
DFS94_RS06625 1237979..1238125 - 147 Protein_1274 transposase domain-containing protein -
DFS94_RS06630 1238179..1238361 + 183 WP_001296208.1 hypothetical protein -
DFS94_RS06635 1238462..1238791 - 330 WP_000450409.1 DUF496 family protein -
DFS94_RS06640 1238963..1240021 - 1059 WP_001200889.1 FUSC family protein -
DFS94_RS06645 1240219..1240692 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
DFS94_RS06650 1240811..1241977 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T105617 WP_000854815.1 NZ_CP029420:1236999-1237373 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T105617 NZ_CP029420:1236999-1237373 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT105617 WP_001280918.1 NZ_CP029420:1236542-1236910 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT105617 NZ_CP029420:1236542-1236910 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References