Detailed information of TA system TA105594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 254651..255235
• Replicon: plasmid pOXA58_010030
• Accession: NZ_CP029396
• Organism: Acinetobacter defluvii strain WCHA30

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: DJ533_RS01940
• Protein ID: WP_000286964.1
• Coordinates: 254651..254953 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DJ533_RS01945
• Protein ID: WP_000985609.1
• Coordinates: 254954..255235 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DJ533_RS01900	250224..251549	+	1326	WP_008942541.1	ISNCY family transposase	-
DJ533_RS01910	251806..252093	+	288	WP_000438825.1	BrnT family toxin	-
DJ533_RS01915	252080..252394	+	315	WP_004728120.1	BrnA antitoxin family protein	-
DJ533_RS01920	252483..253013	-	531	WP_000172759.1	hypothetical protein	-
DJ533_RS01930	253206..254531	+	1326	WP_008942541.1	ISNCY family transposase	-
DJ533_RS01940	254651..254953	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DJ533_RS01945	254954..255235	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
DJ533_RS01950	255315..255734	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
DJ533_RS01955	255874..256194	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DJ533_RS01960	256187..256459	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
DJ533_RS01965	256550..257875	-	1326	WP_081406156.1	ISNCY family transposase	-
DJ533_RS01975	258439..259914	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / sul1 / qacE / ARR-3 / sul2 / blaOXA-58 / msr(E) / mph(E) / cmlB1	-	1..355358	355358
	-	inside	IScluster/Tn	blaOXA-58 / msr(E) / mph(E)	-	233445..262873	29428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T105594 WP_000286964.1 NZ_CP029396:254651-254953 [Acinetobacter defluvii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T105594 NZ_CP029396:254651-254953 [Acinetobacter defluvii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT105594 WP_000985609.1 NZ_CP029396:254954-255235 [Acinetobacter defluvii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT105594 NZ_CP029396:254954-255235 [Acinetobacter defluvii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94