Detailed information of TA system TA105593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 251806..252394
• Replicon: plasmid pOXA58_010030
• Accession: NZ_CP029396
• Organism: Acinetobacter defluvii strain WCHA30

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: DJ533_RS01910
• Protein ID: WP_000438825.1
• Coordinates: 251806..252093 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: DJ533_RS01915
• Protein ID: WP_004728120.1
• Coordinates: 252080..252394 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DJ533_RS01875	247219..247602	+	384	WP_081406170.1	AraC family ligand binding domain-containing protein	-
DJ533_RS01880	247690..248943	+	1254	WP_000182043.1	IS256 family transposase	-
DJ533_RS01885	248970..249434	+	465	Protein_238	helix-turn-helix transcriptional regulator	-
DJ533_RS01890	249484..250089	+	606	WP_000447788.1	LysE family translocator	-
DJ533_RS01900	250224..251549	+	1326	WP_008942541.1	ISNCY family transposase	-
DJ533_RS01910	251806..252093	+	288	WP_000438825.1	BrnT family toxin	Toxin
DJ533_RS01915	252080..252394	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
DJ533_RS01920	252483..253013	-	531	WP_000172759.1	hypothetical protein	-
DJ533_RS01930	253206..254531	+	1326	WP_008942541.1	ISNCY family transposase	-
DJ533_RS01940	254651..254953	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DJ533_RS01945	254954..255235	+	282	WP_000985609.1	putative addiction module antidote protein	-
DJ533_RS01950	255315..255734	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
DJ533_RS01955	255874..256194	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DJ533_RS01960	256187..256459	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / sul1 / qacE / ARR-3 / sul2 / blaOXA-58 / msr(E) / mph(E) / cmlB1	-	1..355358	355358
	-	inside	IScluster/Tn	blaOXA-58 / msr(E) / mph(E)	-	233445..262873	29428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T105593 WP_000438825.1 NZ_CP029396:251806-252093 [Acinetobacter defluvii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T105593 NZ_CP029396:251806-252093 [Acinetobacter defluvii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT105593 WP_004728120.1 NZ_CP029396:252080-252394 [Acinetobacter defluvii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT105593 NZ_CP029396:252080-252394 [Acinetobacter defluvii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9