Detailed information of TA system TA105576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 204341..204984
• Replicon: plasmid pTetD_040074
• Accession: NZ_CP029387
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SCKP040074

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CVG30_RS01565
• Protein ID: WP_014386165.1
• Coordinates: 204568..204984 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CVG30_RS01560
• Protein ID: WP_032408893.1
• Coordinates: 204341..204571 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVG30_RS01530	199414..200409	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
CVG30_RS01535	200416..201564	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
CVG30_RS01540	201735..202892	-	1158	WP_032408895.1	acetylornithine deacetylase	-
CVG30_RS01545	202908..203582	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
CVG30_RS01550	203587..204021	-	435	WP_000103648.1	RidA family protein	-
CVG30_RS01560	204341..204571	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CVG30_RS01565	204568..204984	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
CVG30_RS01570	205307..206275	+	969	WP_074422984.1	IS5 family transposase	-
CVG30_RS01575	206455..206829	-	375	WP_032408891.1	hypothetical protein	-
CVG30_RS01580	206885..207211	-	327	WP_004152639.1	hypothetical protein	-
CVG30_RS01585	207208..207936	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
CVG30_RS01590	207933..208364	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(D)	-	1..226524	226524
	-	flank	IS/Tn	-	-	205352..206275	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T105576 WP_014386165.1 NZ_CP029387:204568-204984 [Klebsiella pneumoniae subsp. pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T105576 NZ_CP029387:204568-204984 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT105576 WP_032408893.1 NZ_CP029387:204341-204571 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT105576 NZ_CP029387:204341-204571 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA