Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 180445..181190 | Replicon | plasmid pTetD_040074 |
| Accession | NZ_CP029387 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain SCKP040074 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A331KSM6 |
| Locus tag | CVG30_RS01420 | Protein ID | WP_032408901.1 |
| Coordinates | 180699..181190 (+) | Length | 164 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | CVG30_RS01415 | Protein ID | WP_014386183.1 |
| Coordinates | 180445..180711 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CVG30_RS01355 | 175997..176410 | - | 414 | WP_013023817.1 | helix-turn-helix domain-containing protein | - |
| CVG30_RS01360 | 176411..176689 | - | 279 | WP_004152721.1 | helix-turn-helix domain-containing protein | - |
| CVG30_RS01365 | 176679..176999 | - | 321 | WP_014386190.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| CVG30_RS01370 | 177080..177304 | - | 225 | WP_014386189.1 | hypothetical protein | - |
| CVG30_RS01375 | 177315..177527 | - | 213 | WP_014386188.1 | hypothetical protein | - |
| CVG30_RS01380 | 177589..177915 | - | 327 | WP_014386187.1 | hypothetical protein | - |
| CVG30_RS01395 | 178552..178902 | - | 351 | WP_014386186.1 | hypothetical protein | - |
| CVG30_RS01400 | 178899..179171 | - | 273 | WP_032408902.1 | hypothetical protein | - |
| CVG30_RS01405 | 179361..179846 | + | 486 | WP_014386185.1 | hypothetical protein | - |
| CVG30_RS01410 | 180090..180248 | - | 159 | WP_014386184.1 | type I toxin-antitoxin system Hok family toxin | - |
| CVG30_RS01415 | 180445..180711 | + | 267 | WP_014386183.1 | DUF1778 domain-containing protein | Antitoxin |
| CVG30_RS01420 | 180699..181190 | + | 492 | WP_032408901.1 | GNAT family N-acetyltransferase | Toxin |
| CVG30_RS01425 | 181631..181882 | - | 252 | WP_032408900.1 | aldo/keto reductase | - |
| CVG30_RS01430 | 182079..183671 | - | 1593 | Protein_194 | IS66 family transposase | - |
| CVG30_RS01435 | 183702..184052 | - | 351 | WP_014386180.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| CVG30_RS01440 | 184049..184489 | - | 441 | WP_014386179.1 | IS66 family insertion sequence hypothetical protein | - |
| CVG30_RS01445 | 184751..185506 | - | 756 | WP_032408898.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(D) | - | 1..226524 | 226524 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 164 a.a. Molecular weight: 17667.46 Da Isoelectric Point: 8.8757
>T105575 WP_032408901.1 NZ_CP029387:180699-181190 [Klebsiella pneumoniae subsp. pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
Download Length: 492 bp
>T105575 NZ_CP029387:180699-181190 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10090.66 Da Isoelectric Point: 5.1169
>AT105575 WP_014386183.1 NZ_CP029387:180445-180711 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
Download Length: 267 bp
>AT105575 NZ_CP029387:180445-180711 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|