Detailed information of TA system TA105548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 96950..97593
• Replicon: plasmid pKPC2_020079
• Accession: NZ_CP029381
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SCKP020079

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: CLQ72_RS00840
• Protein ID: WP_001044770.1
• Coordinates: 96950..97366 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: CLQ72_RS00845
• Protein ID: WP_001261282.1
• Coordinates: 97363..97593 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ72_RS00810	92565..92798	+	234	WP_023148136.1	hypothetical protein	-
CLQ72_RS00815	92836..93738	+	903	WP_002903955.1	NAD(P)-dependent oxidoreductase	-
CLQ72_RS00820	93750..95002	+	1253	Protein_137	four-carbon acid sugar kinase family protein	-
CLQ72_RS00825	94995..95309	+	315	Protein_138	hypothetical protein	-
CLQ72_RS00830	95372..96076	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CLQ72_RS00835	96139..96876	-	738	Protein_140	ATP-dependent endonuclease	-
CLQ72_RS00840	96950..97366	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
CLQ72_RS00845	97363..97593	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CLQ72_RS00850	97577..98011	+	435	WP_015493090.1	hypothetical protein	-
CLQ72_RS00855	98172..99116	+	945	WP_011977810.1	hypothetical protein	-
CLQ72_RS00860	99195..99545	+	351	WP_032738650.1	hypothetical protein	-
CLQ72_RS00865	99603..100124	+	522	WP_013214008.1	hypothetical protein	-
CLQ72_RS00870	100170..100373	+	204	WP_011977813.1	hypothetical protein	-
CLQ72_RS00875	100403..101407	+	1005	WP_011977814.1	hypothetical protein	-
CLQ72_RS00880	101591..102370	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12	-	1..146790	146790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T105548 WP_001044770.1 NZ_CP029381:c97366-96950 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T105548 NZ_CP029381:c97366-96950 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT105548 WP_001261282.1 NZ_CP029381:c97593-97363 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT105548 NZ_CP029381:c97593-97363 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3