Detailed information of TA system TA105547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3758..4338
• Replicon: plasmid p1_020079
• Accession: NZ_CP029379
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SCKP020079

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CLQ72_RS00040
• Protein ID: WP_071177730.1
• Coordinates: 4024..4338 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CLQ72_RS00035
• Protein ID: WP_000093040.1
• Coordinates: 3758..4036 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ72_RS00010	199..1683	-	1485	WP_004152765.1	group II intron reverse transcriptase/maturase	-
CLQ72_RS00015	1683..1934	-	252	WP_009483812.1	hypothetical protein	-
CLQ72_RS00020	2331..2576	+	246	WP_032440458.1	hypothetical protein	-
CLQ72_RS00025	2850..3221	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
CLQ72_RS00030	3218..3424	+	207	Protein_5	TonB family protein	-
CLQ72_RS00035	3758..4036	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CLQ72_RS00040	4024..4338	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ72_RS00045	4502..4930	+	429	WP_001140599.1	hypothetical protein	-
CLQ72_RS00050	4956..5135	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CLQ72_RS00055	5162..5692	-	531	WP_071177729.1	hypothetical protein	-
CLQ72_RS00060	5699..6430	-	732	WP_071177728.1	replication-relaxation family protein	-
CLQ72_RS00065	6430..8394	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-
CLQ72_RS00070	8422..8727	-	306	WP_071994323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T105547 WP_071177730.1 NZ_CP029379:4024-4338 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T105547 NZ_CP029379:4024-4338 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT105547 WP_000093040.1 NZ_CP029379:3758-4036 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT105547 NZ_CP029379:3758-4036 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1