Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3782194..3782848 Replicon chromosome
Accession NZ_CP029371
Organism Escherichia coli strain C

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag DIZ83_RS19685 Protein ID WP_000244783.1
Coordinates 3782441..3782848 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DIZ83_RS19680 Protein ID WP_000354046.1
Coordinates 3782194..3782460 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DIZ83_RS19655 3777363..3778106 + 744 WP_000951948.1 SDR family oxidoreductase -
DIZ83_RS19660 3778163..3779596 - 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
DIZ83_RS19665 3779641..3779952 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
DIZ83_RS19670 3780116..3780775 + 660 WP_000250274.1 hemolysin III family protein -
DIZ83_RS19675 3780971..3781951 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DIZ83_RS19680 3782194..3782460 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DIZ83_RS19685 3782441..3782848 + 408 WP_000244783.1 protein YgfX Toxin
DIZ83_RS19690 3782888..3783409 - 522 WP_001055874.1 flavodoxin FldB -
DIZ83_RS19695 3783521..3784417 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DIZ83_RS19700 3784442..3785152 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DIZ83_RS19705 3785158..3786891 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T105545 WP_000244783.1 NZ_CP029371:3782441-3782848 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T105545 NZ_CP029371:3782441-3782848 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT105545 WP_000354046.1 NZ_CP029371:3782194-3782460 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT105545 NZ_CP029371:3782194-3782460 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References