Detailed information of TA system TA105538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1691440..1692277
• Replicon: chromosome
• Accession: NZ_CP029371
• Organism: Escherichia coli strain C

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: DIZ83_RS08990
• Protein ID: WP_000227786.1
• Coordinates: 1691735..1692277 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: DIZ83_RS08985
• Protein ID: WP_001297137.1
• Coordinates: 1691440..1691751 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIZ83_RS08960	1686460..1687407	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
DIZ83_RS08965	1687429..1689420	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
DIZ83_RS08970	1689410..1690024	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
DIZ83_RS08975	1690024..1690353	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
DIZ83_RS08980	1690365..1691255	+	891	WP_000971336.1	heme o synthase	-
DIZ83_RS08985	1691440..1691751	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
DIZ83_RS08990	1691735..1692277	+	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
DIZ83_RS08995	1692333..1693268	-	936	WP_001297127.1	sel1 repeat family protein	-
DIZ83_RS09000	1693685..1695049	+	1365	WP_001000978.1	MFS transporter	-
DIZ83_RS09005	1695177..1695668	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
DIZ83_RS09010	1695836..1696747	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T105538 WP_000227786.1 NZ_CP029371:1691735-1692277 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T105538 NZ_CP029371:1691735-1692277 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT105538 WP_001297137.1 NZ_CP029371:1691440-1691751 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT105538 NZ_CP029371:1691440-1691751 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3