Detailed information of TA system TA105526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1434242..1434850
• Replicon: chromosome
• Accession: NZ_CP029370
• Organism: Paenibacillus polymyxa strain CJX518

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: DI243_RS06325
• Protein ID: WP_007429335.1
• Coordinates: 1434500..1434850 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DI243_RS06320
• Protein ID: WP_170970806.1
• Coordinates: 1434242..1434493 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI243_RS06305	1430433..1431065	+	633	WP_040101871.1	LysE family transporter	-
DI243_RS06310	1431176..1432453	+	1278	WP_040101870.1	outer membrane lipoprotein carrier protein LolA	-
DI243_RS06315	1432722..1433918	+	1197	WP_013369924.1	alanine racemase	-
DI243_RS06320	1434242..1434493	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
DI243_RS06325	1434500..1434850	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DI243_RS06330	1435029..1437251	+	2223	WP_029514917.1	RNA-binding transcriptional accessory protein	-
DI243_RS06335	1437356..1437490	-	135	WP_013369928.1	cortex morphogenetic protein CmpA	-
DI243_RS06340	1437634..1438146	+	513	WP_013369929.1	SprT family protein	-
DI243_RS06355	1438479..1439354	-	876	WP_040101869.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T105526 WP_007429335.1 NZ_CP029370:1434500-1434850 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T105526 NZ_CP029370:1434500-1434850 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT105526 WP_170970806.1 NZ_CP029370:1434242-1434493 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT105526 NZ_CP029370:1434242-1434493 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1