Detailed information of TA system TA105512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1341730..1342355
• Replicon: chromosome
• Accession: NZ_CP029369
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DI496_RS08590
• Protein ID: WP_000911330.1
• Coordinates: 1341957..1342355 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: DI496_RS08585
• Protein ID: WP_000450524.1
• Coordinates: 1341730..1341957 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS08560	1337533..1338003	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
DI496_RS08565	1338003..1338575	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
DI496_RS08570	1338721..1339599	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
DI496_RS08575	1339616..1340650	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
DI496_RS08580	1340863..1341576	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
DI496_RS08585	1341730..1341957	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
DI496_RS08590	1341957..1342355	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DI496_RS08595	1342502..1343365	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
DI496_RS08600	1343380..1345395	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
DI496_RS08605	1345469..1346167	+	699	WP_000679823.1	esterase	-
DI496_RS08610	1346277..1346477	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T105512 WP_000911330.1 NZ_CP029369:1341957-1342355 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T105512 NZ_CP029369:1341957-1342355 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT105512 WP_000450524.1 NZ_CP029369:1341730-1341957 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT105512 NZ_CP029369:1341730-1341957 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33