Detailed information of TA system TA105494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 60936..61537
• Replicon: plasmid p1_035148
• Accession: NZ_CP029365
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DI496_RS00330
• Protein ID: WP_112031922.1
• Coordinates: 60936..61316 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: DI496_RS00335
• Protein ID: WP_001190712.1
• Coordinates: 61316..61537 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI496_RS00300	57057..57365	+	309	WP_000349257.1	hypothetical protein	-
DI496_RS00305	57362..57838	+	477	WP_000861173.1	hypothetical protein	-
DI496_RS00310	57835..58329	+	495	WP_112031919.1	dUTP diphosphatase	-
DI496_RS00315	58344..59045	+	702	WP_089477112.1	DUF2829 domain-containing protein	-
DI496_RS00320	59052..59816	+	765	WP_112031920.1	hypothetical protein	-
DI496_RS00325	59806..60900	+	1095	WP_112031921.1	hypothetical protein	-
DI496_RS00330	60936..61316	-	381	WP_112031922.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DI496_RS00335	61316..61537	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DI496_RS00340	61610..61999	-	390	WP_000506731.1	hypothetical protein	-
DI496_RS00345	62098..62349	-	252	WP_112031923.1	DNA polymerase III subunit theta	-
DI496_RS00355	62664..63494	-	831	WP_112031924.1	DUF551 domain-containing protein	-
DI496_RS00360	63505..63768	-	264	WP_000224218.1	hypothetical protein	-
DI496_RS00365	63770..63961	-	192	WP_021517457.1	hypothetical protein	-
DI496_RS00370	64134..64580	-	447	WP_112031925.1	ead/Ea22-like family protein	-
DI496_RS00375	64577..65263	-	687	WP_080028522.1	ead/Ea22-like family protein	-
DI496_RS00380	65250..65939	-	690	WP_112031926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89248	89248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13637.50 Da
• Isoelectric Point: 5.6342

>T105494 WP_112031922.1 NZ_CP029365:c61316-60936 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRDLYGSNI

Nucleotide

• Length: 381 bp

>T105494 NZ_CP029365:c61316-60936 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGACCTATATGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT105494 WP_001190712.1 NZ_CP029365:c61537-61316 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT105494 NZ_CP029365:c61537-61316 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAAGTTGA
AATCACCCGCAGAGGCCGTGAACCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAGGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6